Recombinant Human TNF-α

TNF-α是pleiotrophic促炎细胞因子分泌的多种细胞包括脂肪细胞,激活单核细胞、巨噬细胞、B细胞、T细胞和成纤维细胞。它属于TNF家族的配体和信号通过两个受体,TNFR1 TNFR2。TNF-α是各种肿瘤细胞和细胞毒性是一个重要的中介因素对细菌感染的免疫反应。TNF-α也扮演了重要的角色在感染性休克的感应,自动免疫疾病、风湿性关节炎、炎症和糖尿病。

一.产品名称：Recombinant Human TNF-α/Peprotech  TNF-α

二.Catalog Number:AF-300-01A

三.Synonyms:

Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

四.Description:

TNF-α is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. Manufactured using all non-animal reagents.

五.AA Sequence:

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

六.Source:E.coli

七.Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.

八.Biological Activity:

The ED₅₀as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.

九.Cross Reactivity:Cow, Monkey, Mouse, Rat, Sheep, Human, Dog, Pig, N/A, Human + Virus