Recombinant Human FGF-basic （154 a.a.）

FGF-basic是23已知FGF家族的成员之一。这个家庭中发挥核心作用的蛋白质在产前发展和产后的生长和再生各种组织,通过促进细胞增殖和分化。FGF-basic non-glycosylated肝素结合生长因子,表示在大脑中,垂体,肾、视网膜、骨头、睾丸、肾上腺肝、单核细胞、上皮细胞和内皮细胞。重组人类FGF-basic 17.2 kDa蛋白由154个氨基酸残基组成。使用所有无动物生产试剂。

一.产品名称：Recombinant Human FGF-basic

二.货号Catalog Number:AF-100-18B

三.同义Synonyms:Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

四.描述Description:

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues. Manufactured using all non-animal reagents.

五.AA序列AA Sequence:

AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

六.来源Source:E.coli

七.纯度Purity:Greater than 95% by SDS-PAGE gel and HPLC analyses.

八.生物活性Biological Activity:

Assay #1: The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5ng/ml corresponding to a specific activity of ≥ 2 x 10⁶ units/mg.

Assay #2: The ED₅₀ was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.

九.交叉性Cross Reactivity:

Cow, Dog, Frog, Hamster, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Human + Mouse, Rat + Chicken, Human, Chicken, Horse, N/A, Human + Virus

十.产地：美国Peprotech