Arginase-1 精氨酸酶1（兔单克隆抗体）

Arginase-1 精氨酸酶1（兔单克隆抗体）

广州健仑生物科技有限公司

雄激素中zui主要的形式为睾酮，雄激素在体内起着重要的作用。除了与生植相关外，还具有保持体内激素平衡；刺激蛋白质合成代谢，促进氮沉积和增加肌纤维的数量和厚度等。通过对青春期前的猪（体重约为15kg）和青春期的猪（体重约为74kg）分组试验，结果表明公猪的饲料报酬率比去势公猪高20%，用睾酮或二氢睾酮处理的去势公猪，蛋白质合成率显着高于对照去势公猪，蛋白质降解率显着低于对照去势公猪（Mulvaney，1984）。James T等（1992）通过用去甲雄三烯醇酮乙酸盐处理小公牛，结果发现经处理的小公牛平均日增重高于对照小公牛（P<O.001）。Tarocco C等（1986）对63头大白公猪从30到130kg活重进行表型检测发现血液中睾酮浓度与背膘呈显着相关。然而雄激素是通过与雄激素受体（Androgen Receptor-AR）结合而发挥其功能的，若无雄激素受体，则雄激素对组织无刺激反应（refractory）。雄激素受体是雄激素作用的中介物质。许多年来欧美一些国家一直在研究雄激素受体基因。

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【产品介绍】

细胞定位：细胞浆/细胞核

克隆号：SP156

同型：IgG

适用组织：石蜡/冰冻

阳性对照：正常肝脏/肝细胞癌

抗原修复：热修复（EDTA）

抗体孵育时间：60min

Arginase-1 精氨酸酶1（兔单克隆抗体）

生物学特性
1998年Schellekens和Girbal Neuhause等根据filaggrin的cDNA序列合成的多肽证实瓜氨酸残基是类风湿关节炎特异的抗中间丝相关蛋白（filaggrin）抗体识别表位的必需组成。通过对基因文库中各个序列号的filggrin氨基酸序列进行分析，合成了一条以瓜氨酸代替精氨酸的20个左右氨基酸残基的肽链。并通过一定的试验显示了瓜氨酸是RA患者血清中抗filaggrin相关抗体识别的主要组成性抗原决定簇成分。在2000年，Schellekens将一条由19个氨基酸残基组成的瓜氨酸肽链中的两个丝氨酸替换为半胱氨酸，形成与β-转角具有相似结构的二硫键，合成环瓜氨酸肽（cyclic citrullinated peptide,CCP）。采用环瓜氨酸肽为抗原基质用ELISA法检测RA患者血清中的抗环瓜氨酸肽抗体，敏感性和特异性均有明显提高。
研究简史
抗环瓜氨酸肽抗体（CCP）ELISA法是1998年Schellkens等建立起来辅助诊断RA的检测方法，该技术一出现，立即备受关注，现已有商品供应。按照前丝集蛋白cDNA序列，人工合成可能具有抗原表位的9~19个氨基酸短肽，并经胍氨酸化修饰，经ELISA筛选出具有抗原性片段。
检测方法
应用ELISA法可定性或定量检测患者血清中抗CCP抗体。Jansen AL等通过不同类型的多关节疾病患者血清中抗CCP抗体的测定，预测了抗CCP抗体大于50AU/ml即可对早期类风湿性关节炎有较高的敏感性和特异性。检测了RA患者抗CCP抗体的水平，结果也同样显示了抗CCP抗体的临界值为50AU/ml。并且RA患者清血清中抗CCP抗体的平均水平为1100AU/ml*（范围为：57～3419AU/ml。而对照组抗CCP抗体的平均水平仅为6.8AU/ml（范围为：1～39AU/ml）。进一步证实了抗CCP抗体对RA患者的诊断价值。

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

Biological characteristics
The 1998 polypeptide synthesized by Schellekens and Girbal Neuhause, based on the cDNA sequence of filaggrin, demonstrated that citrulline residues are essential components of rheumatoid arthritis-specific anti-filaggrin antibody epitope recognition. By analyzing the amino acid sequence of filggrin of each sequence number in the gene library, a peptide chain of about 20 amino acid residues with citrulline instead of arginine was synthesized. And through certain tests showed that citrulline is the major constitutive antigenic determinant of anti-filaggrin-associated antibody in serum of RA patients. In 2000, Schellekens replaced two serines in a citrulline peptide chain consisting of 19 amino acid residues with cysteine ​​to form disulfide bonds with a similar structure to β-turn, and synthesized cyclic citrulline Cyclic citrullinated peptide (CCP). Using cyclic citrullinated peptide as antigen matrix, the anti-cyclic citrullinated peptide antibody in serum of patients with RA was detected by ELISA, the sensitivity and specificity were significantly improved.
Brief history of the study
The anti-cyclic citrullinated peptide antibody (CCP) ELISA method was established in 1998 by Schellkens et al to assist in the diagnosis of RA. As soon as the technique became available, there was immediate concern that the current supply of the product was available. According to the pre-silk cDNA sequence, artificial short peptides of 9 to 19 amino acids which may have antigenic epitopes were synthesized and modified by guanidine. Antigenic fragments were selected by ELISA.
Detection method
Anti-CCP antibodies in serum of patients can be detected qualitatively or quantitatively by ELISA. Jansen AL and other anti-CCP antibodies in serum of patients with different types of multi-joint diseases, the anti-CCP antibody predicted greater than 50AU / ml early rheumatoid arthritis can have a higher sensitivity and specificity. The level of anti-CCP antibodies in RA patients was also tested and the results also showed that the cutoff value of anti-CCP antibody was 50 AU / ml. The average level of anti-CCP antibody in serum of RA patients was 1100 AU / ml * (range: 57-3419 AU / ml) while the average level of anti-CCP antibody in control group was only 6.8 AU / ml ml), which further confirmed the diagnostic value of anti-CCP antibody in patients with RA.