| Designations: | GH3 |
| Depositors: | AH Tashjian |
| Biosafety Level: | 1 |
| Shipped: | frozen |
| Medium & Serum: | See Propagation |
| Growth Properties: | loosely adherent with floating clusters |
| Organism: | Rattus norvegicus （rat） |
| Morphology: | epithelial
|
| Source: | Organ: pituitary
Strain: Wistar-Furth
Disease: tumor |
| Cellular Products: | prolactin; growth hormone （somatotrophin） |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| |
| Isolation: | Isolation date: July, 1965 |
| Applications: | transfection host （Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents） |
| Virus Susceptibility: | Herpes simplex virus
Vesicular stomatitis virus
Human poliovirus 1 |
| Reverse Transcript: | negative |
| Cytogenetic Analysis: | modal number = 67; range = 47 to 71.
Stemline karyotype is stable with a few structural alterations. Two dicentric marker chromosomes were observed in 100% of the cells examined. |
| Age: | 7 months |
| Gender: | female |
| Comments: | Clone GH3 was established in July, 1965 by A.H. Tashjian, Jr., et al. from a pituitary tumor carried in a 7-month-old female Wistar-Furth rat [881]
Although the GH3 was not a direct derivative of the GH1 clone （ATCC CCL-82）, it was initiated after only two additional animal passages from the primary culture from which the GH1 clone was established.
The epithelial-like GH3 clone generates growth hormone at a greater rate than the GH1 cells and also produces prolactin.
Studies on the control of the production of these protein hormones by the GH3 cells have shown that hydrocortisone stimulates the production of growth hormone and inhibits prolactin production.
The cells have been adapted to growth in suspension culture using Eagle's minimum essential medium （spinner） supplemented with 15% horse serum and 2.5% FBS. Under these conditions the cells continue to produce both growth hormone and prolactin. [884]
The cell line was submitted to the American Type Culture Collection in October, 1972 in the 10th subculture generation. |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C |
QQ交谈
