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化工仪器网>产品展厅>医疗器械设备>血液学分析设备>流式细胞仪>Deformability Cytometry Real-Time Deformability Cytometry

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Deformability Cytometry Real-Time Deformability Cytometry

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世联博研(北京)科技有限公司(Bio Excellence International Tech Co.,Ltd)简称为世联博研。世联博研是一家集进口科研仪器代理销售以及实验技术服务于一体的技术公司。世联博研专注生物力学和3D生物打印前沿科研设备代理销售及科研实验项目合作服务,内容涵盖了血管力学生物学、生物力学建模仿真与应用、细胞分子生物力学、组织修复生物力学、骨与关节生物力学、口腔力学生物学、眼耳鼻咽喉生物力学、康复工程生物力学、生物材料力学与仿生学、人体运动生物力学等生物力学研究以及生物材料打印、打印样品生物力学性能测试分析的前沿领域科研利器和科研服务。

世联博研的客户范围:
科研院所单位、生物医学科研高校、医院基础科研单位等。

世联博研公司代理的品牌具有:
1)近10年长期稳定的货源
2)以生物力学、细胞力学、细胞生物分子学、生物医学组织工程、生物材料学为主,兼顾其他相关产品线
3)提供专业产品培训和销售培训
4)良好的技术支持
5)已成交老客户考证
6)每年新增的货源。

细胞应力加载仪,3细胞打印机,NanoTweezer新型激光光镊系统,PicoTwist磁镊,美国NeuroIndx品牌Kuiqpick单细胞捕获切割系统

产地类别 进口 产品种类 细胞分析系统
价格区间 面议 应用领域 医疗卫生,生物产业

细胞实时形变测量分析系统,Real-Time Deformability Cytometry

来自德国的高通量单细胞形变测量分析系统

 

 

该系统是一套基于微流控流体压力梯度的、在倒置显微镜的扩展起来的、集成流式细胞仪特性、荧光检测模块、温控模 块、高速成像和数据采集分析软件的高通量单细胞实时形变测量和单细胞力学性质分析系统。
     是一种以流式细胞仪的速度检测单个细胞形态和力学性质的技术,每个细胞都被实时拍摄、分析和成像存储。 此外,非破坏性的力量应用于细胞,提供一种方便,稳健和高通量的技术进行生物标志物的检测,可用于基础科学和临床研究。

探索细胞的物理特性作为生物标志物,可以将非破坏性的力量应用于细胞或珠子,并观察它们的变形。 这允许研究对物理压力的特定机械响应。

优势亮点:

  • 机械力学作为一种新的生物标志物--温和无损伤
  • 无标记
  • 非破坏性的力量
  • 高速测量单个细胞的实时形变(高度、宽度、面积、比例)、高度偏差、环度衡量比、孔度、亮度、杨氏模量等
  • 细胞机械特性测量高通量(1000细胞/秒)
  • 配有高速成像、荧光检测、温控模块
  • 不需要细胞分离/纯化
  • 文献量大、级别高文章达数十篇

实时形变

 

成像

每个细胞被同时拍照、分析和储存。 这允许通过它们的光学特性来找到小亚群或区分细胞。 另外可以研究像表面拓扑或细胞对光的衰减的形态特性。

  • 每个获取图像的存储
  • 快速访问细胞大小和形态

该高速流式细胞形变机械力学测量系统是一种以细胞计数器的速度检测单细胞形态和流变性质的技术! 细胞被泵送通过微流控芯片。 每个细胞都被实时拍摄、分析和成像存储。 此外,非破坏性的力量应用于细胞,提供一种方便,稳健和高通量的技术进行生物标志物的检测,可用于基础科学和临床研究。

 

流式细胞技术

流式单细胞力学特性测试分析系统

 

细胞通过微流通道时,提取细胞变形、亮度和大小等参数,同时。 这允许实时地研究细胞属性。

  • 可温控和荧光检测

    实时变形细胞计数和同时荧光检测:

  •   荧光模块使得该系统不再只是附加了一个额外的细胞力学检测通道的流式细胞仪。它成为了生命科学实验室的得力工具 - 提供了更多视角来解决科学问题。在生物学研究中通常使用荧光流式细胞仪来鉴定和定量细胞和细胞过程。使该系统集荧光流式细胞仪和实时变形的优点于一身,形成了实时荧光形变细胞仪。光片激发设计可实现三通道1D荧光成像。除了所有实时变形参数外,系统还会分析荧光信号实时得到峰图,速度可达每秒1000个细胞。也可在实验后处理保存的原始荧光数据,以针对特定的问题和需 求修改处理方法。

  • 1)根据表面marker鉴定血细胞:
    荧光模块可检测和鉴定同一样品中的三种不同荧光。利用标记的表面荧光蛋白可同时实现细胞鉴定和力学性质及形态性质测量。 下图为 G-CSF动员的外周血样品细胞群体。 标记后的细胞表面markers CD3-FITC (T-cells), CD34-PE (造血干细胞)和CD14-APC(单核细胞)荧光强度检测 揭示了各细胞类型所具有的不同力学性质。 
  • 2)一维荧光成像:
    荧光模块在激发光路径中产生一束受限光片,穿过流道,细胞会经过一束很窄的激发光幕。这样可以进行1D荧光成像,例如可用于解析沿流动方向的荧光标记结构的侧向分布。检测到的荧光峰值带有很多重要信息。荧光标记的胞内结构(如细胞核)会显示窄峰,而胞质会显示出更宽的峰。不同分裂期细胞中标记的组蛋白也会呈现出不同的峰图. 

  • 加热模块 - 温度控制
    加热模块实现了生理温度下的测量。加热模块带有一个300 W的加热器和几个静默通风机来有效混合热空气。靠近样品处有一个传感器和一个控制单元,用以地将温度控制在所需值。系统的空气循环系统非常高效,当进行开放操作(如更换样品)后可以迅速恢复温度。 
    高速摄像
    该成像模块是款高速明场摄像显微镜,使用同步化微秒高强度LED光源减轻运动模糊,可进行极慢运动摄影.每秒可记录500幅全帧图像或10000帧小区域图像

越来越多的研究表明,机械力(Mechanical Forces)是机体生长发育、结构重建以及功能维持的重要因素,也与疾病的发生发展密切相关。力学刺激(Mechanical Stimulation)会对细胞的粘附、铺展、迁移、增殖和分化等行为有着重要的影响,力学表型(Mechanical phenotype)的改变也可用来定量检测、评价细胞的生物学功能。力学生物学(Mechnobiology)研究对深入揭示疾病机理、发展新的诊断治疗方法具有重要意义,还可以为生物材料、再生医学、药物筛选等应用研究提供新思路、新工具。由于细胞内在的生物复杂性及微纳米尺度的制约,如何实现细胞及亚细胞水平上力学信息的实时、原位、动态探测和操控是力学生物学中的挑战性的问题之一。

 

 

Zebrafish spinal cord repair is accompanied by transient tissue stiffening

S Möllmert, MA Kharlamova, T Hoche, AV Taubenberger, S Abuhattum, ...

Biophysical Journal

22019
Polyacrylamide bead sensors for in vivo quantification of cell-scale stress in zebrafish development

N Traeber, K Uhlmann, S Girardo, G Kesavan, K Wagner, J Friedrichs, ...

Scientific reports 9 (1), 1-14

32019
CASP1 variants influence subcellular caspase-1 localization, pyroptosome formation, pro-inflammatory cell death and macrophage deformability

F Kapplusch, F Schulze, S Rabe-Matschewsky, S Russ, M Herbig, ...

Clinical Immunology 208, 108232

 2019
Morpho‐Rheological Fingerprinting of Rod Photoreceptors Using Real‐Time Deformability Cytometry

T Santos‐Ferreira, M Herbig, O Otto, M Carido, MO Karl, S Michalakis, ...

Cytometry Part A 95 (11), 1145-1157

 2019
3D microenvironment stiffness regulates tumor spheroid growth and mechanics via p21 and ROCK

AV Taubenberger, S Girardo, N Träber, E Fischer‐Friedrich, M Kräter, ...

Advanced Biosystems 3 (9), 1900128

22019
Controlling distinct signaling states in cultured cancer cells provides a new platform for drug discovery

SW Poser, O Otto, C Arps-Forker, Y Ge, M Herbig, C Andree, ...

The FASEB Journal 33 (8), 9235-9249

12019
Erratum: Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation.

S Tietze, M Kräter, A Jacobi, A Taubenberger, M Herbig, R Wehner, ...

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 6 (8), 1900622-1900622

1*2019
Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation

S Tietze, M Kräter, A Jacobi, A Taubenberger, M Herbig, R Wehner, ...

Advanced Science 6 (8), 1802104

12019
Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry

A Jacobi, P Rosendahl, M Kräter, M Urbanska, M Herbig, J Guck

Stem Cell Mobilization, 135-148

 2019
Buckling of epithelium growing under spherical confinement

A Trushko, I Di Meglio, A Merzouki, C Blanch-Mercader, S Abuhattum, ...

bioRxiv, 513119

22019
Oncogenic signaling alters cell shape and mechanics to facilitate cell division under confinement.

HK Matthews, S Ganguli, K Plak, AV Taubenberger, M Piel, J Guck, ...

bioRxiv, 571885

 2019
The Relationship Between Metastatic Potential and In Vitro Mechanical Properties of Osteosarcoma Cells

CN Holenstein, A Horvath, B Schär, AD Schoenenberger, M Bollhalder, ...

Molecular Biology of the Cell 30 (7), 887-898

22019
Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming

KR Bashant, A Vassallo, C Herold, R Berner, L Menschner, ...

Journal of Leukocyte Biology, e12967

62019
Droplet-Assisted Microfluidic Fabrication and Characterization of Multifunctional Polysaccharide Microgels Formed by Multicomponent Reactions

N Hauck, N Seixas, S Centeno, R Schlüßler, G Cojoc, P Müller, J Guck, ...

Polymers 10 (10), 1055

122018
Mechanical mapping of spinal cord growth and repair in living zebrafish larvae by Brillouin imaging

R Schlüßler, S Möllmert, S Abuhattum, G Cojoc, P Müller, K Kim, C Möckel, ...

Biophysical journal 115 (5), 911-923

312018
Comparative study of cell mechanics methods

PH Wu, DRB Aroush, A Asnacios, WC Chen, ME Dokukin, BL Doss, ...

Nature methods 15 (7), 491

 2018
A comparison of methods to assess cell mechanical properties

PH Wu, DRB Aroush, A Asnacios, WC Chen, ME Dokukin, BL Doss, ...

Nature Methods 15 (7), 491-498

752018
Response to Comment on “Cell nuclei have lower refractive index and mass density than cytoplasm”

P Müller, J Guck

Journal of Biophotonics 11 (6), e201800095

12018
Real-time fluorescence and deformability cytometry

P Rosendahl, K Plak, A Jacobi, M Kraeter, N Toepfner, O Otto, C Herold, ...

Nature Methods 15 (5), 355-358

312018
Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging

P Müller, M Schürmann, S Girardo, G Cojoc, J Guck

Optics Express 26 (8), 10729-10743

52018
Toll-like receptor-mediated upregulation of CXCL16 in psoriasis orchestrates neutrophil activation

S Steffen, S Abraham, M Herbig, F Schmidt, K Blau, S Meisterfeld, ...

Journal of Investigative Dermatology 138 (2), 344-354

82018
Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood

N Toepfner, C Herold, O Otto, P Rosendahl, A Jacobi, M Kräter, ...

eLife 7, e29213

332018
High-throughput microfluidic characterization of erythrocyte shape and mechanical variability

F Reichel, J Mauer, AA Nawaz, G Gompper, J Guck, DA Fedosov

bioRxiv, 488189

 2018
Alterations in Cell Mechanics by Actin Cytoskeletal Changes Correlate with Strain-Specific Rubella Virus Phenotypes for Cell Migration and Induction of Apoptosis

M Kräter, J Sapudom, N Bilz, T Pompe, J Guck, C Claus

Cells 7 (9), 136

22018
Acute but not inherited demyelination in mouse models leads to brain tissue stiffness changes

D Eberle, G Fodelianaki, T Kurth, A Jagielska, S Moellmert, E Ulbricht, ...

bioRxiv, 449603

32018
Axonal transport, phase-separated compartments and neuron mechanics—a new approach to investigate neurodegenerative diseases

M Nötzel, G Rosso, S Möllmert, A Seifert, R Schlüßler, K Kim, A Hermann, ...

Frontiers in Cellular Neuroscience 12, 358

22018
High-throughput single-cell mechanical phenotyping with real-time deformability cytometry.

M Urbanska, P Rosendahl, M Kräter, J Guck

Methods in Cell Biology 147, 175-198

62018
Metabolic profiling of human eosinophils

L Porter, N Toepfner, KR Bashant, J Guck, M Ashcroft, N Farahi, ...

Frontiers in Immunology 9, 1404

42018
Standardized microgel beads as elastic cell mechanical probes

S Girardo, N Traeber, K Wagner, G Cojoc, C Herold, R Goswami, ...

Journal of Materials Chemistry B

212018
Intracellular mass density increase is accompanying but not sufficient for stiffening and growth arrest of yeast cells

S Abuhattum, K Kim, TM Franzmann, A Esslinger, D Midtvedt, ...

Frontiers in Physics 6, 39-50

32018
Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells

M Herbig, M Kräter, K Plak, P Müller, J Guck, O Otto

Flow Cytometry Protocols – Methods in Molecular Biology 1678, 347-369

192018
Mechanical mapping of spinal cord development and repair in living zebrafish larvae using Brillouin microscopy

R Schlüßler, S Möllmert, S Abuhattum, G Cojoc, P Müller, K Kim, C Möckel, ...

bioRxiv, 181560

32018
Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage

M Urbanska, M Winzi, K Neumann, S Abuhattum, P Rosendahl, P Müller, ...

Development 144 (23), 4313-4321

42017
Three-dimensional correlative single‐cell imaging utilizing fluorescence and refractive index tomography

M Schürmann, G Cojoc, S Girardo, E Ulbricht, J Guck, P Müller

Journal of Biophotonics 81 (2), e201700145

362017
Method for diagnosis and/or prognosis of cancers by analysis of the mechanical properties of tumor cells

J Käs, J Guck

US Patent 9,683,982

 2017
3D single-cell imaging using combined fluorescence and refractive index tomography

M Schürmann, G Cojoc, S Girardo, E Ulbricht, J Guck, P Müller

arXiv preprint arXiv:1706.00715

 2017
Mechanical deformation induces depolarization of neutrophils

AE Ekpenyong, N Toepfner, C Fiddler, M Herbig, W Li, G Cojoc, ...

Science Advances 3 (6), e1602536

242017
Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells

S Tavares, AF Vieira, AV Taubenberger, M Araújo, NP Martins, ...

Nature Communications 8, 15237

452017
Mechanical strain promotes oligodendrocyte differentiation by global changes of gene expression

A Jagielska, AL Lowe, E Makhija, L Wroblewska, J Guck, RJM Franklin, ...

Frontiers in cellular neuroscience 11, 93

252017
Initiation of acute graft-versus-host disease by angiogenesis

K Riesner, Y Shi, A Jacobi, M Kraeter, M Kalupa, A McGearey, S Mertlitz, ...

Blood 129 (14), 2021-2032

232017
Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion

M Koch, KE Wright, O Otto, M Herbig, ND Salinas, NH Tolia, TJ Satchwell, ...

Proceedings of the National Academy of Sciences 114 (16), 4225-4230

262017
High‐throughput cell mechanical phenotyping for label‐free titration assays of cytoskeletal modifications

S Golfier, P Rosendahl, A Mietke, M Herbig, J Guck, O Otto

Cytoskeleton 74 (8), 283-296

212017
Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase

CJ Chan, W Li, G Cojoc, J Guck

Biophysical Journal 112 (6), 1063-1076

102017
V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling-a new option for HCC therapy

K Bartel, M Winzi, M Ulrich, A Koeberle, D Menche, O Werz, R Müller, ...

Oncotarget 8 (6), 9476

172017
Numerical simulation of real-time deformability cytometry to extract cell mechanical properties

M Mokbel, D Mokbel, A Mietke, N Traber, S Girardo, O Otto, J Guck, ...

ACS Biomaterials Science & Engineering 3 (11), 2962-2973

472017
Real-time fluorescence and deformability cytometry-flow cytometry goes mechanics

P Rosendahl, K Plak, A Jacobi, M Kraeter, N Toepfner, O Otto, C Herold, ...

bioRxiv, 187435

22017
Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion

A Surcel, ES Schiffhauer, D Thomas, Q Zhu, K DiNapoli, M Herbig, O Otto, ...

bioRxiv, 190553

52017
Roadmap on optofluidics

P Minzioni, R Osellame, C Sada, S Zhao, F Omenetto, K Gylfason, ...

Journal of Optics 19 (9), 093003

302017
Bone marrow niche-mimetics modulate HSPC function via integrin signaling

M Kräter, A Jacobi, O Otto, S Tietze, K Müller, DM Poitz, S Palm, VM Zinna, ...

Scientific Reports 7 (1), 2549

122017
Detection Of Human Disease Conditions By Single-Cell Morpho-Rheological Phenotyping Of Whole Blood

N Toepfner, C Herold, O Otto, P Rosendahl, A Jacobi, M Krater, ...

bioRxiv, 145078

32017
Mechanical mismatch between Ras transformed and untransformed epithelial cells

C Gullekson, G Cojoc, M Schürmann, J Guck, A Pelling

Soft Matter 13 (45), 8483-8491

32017
Enlightening discriminative network functional modules behind Principal Component Analysis separation in differential-omic science studies

S Ciucci, Y Ge, C Durán, A Palladini, V Jiménez-Jiménez, ...

Scientific Reports 7, 43946

262017
Niche WNT5A regulates the actin cytoskeleton during regeneration of hematopoietic stem cells

C Schreck, R Istvánffy, C Ziegenhain, T Sippenauer, F Ruf, L Henkel, ...

Journal of Experimental Medicine 214 (1), 156-181

132016
Initiation of Acute Graft-Versus-Host Disease By Angiogenesis

K Riesner, Y Shi, A Jacobi, M Kraeter, M Kalupa, A McGearey, S Mertlitz, ...

Blood, The Journal of the American Society of Hematology 128 (22), 4533-4533

 2016
Chemotherapy impedes in vitro microcirculation and promotes migration of leukemic cells with impact on metastasis

SV Prathivadhi-Bhayankaram, J Ning, M Mimlitz, C Taylor, E Gross, ...

Biochemical and Biophysical Research Communications 479 (4), 841-846

52016
Brain tissue stiffness is a sensitive marker for acidosis

K Holtzmann, HOB Gautier, AF Christ, J Guck, RT Káradóttir, K Franze

Journal of Neuroscience Methods 271, 50-54

172016
The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512

H Mziaut, B Mulligan, P Hoboth, O Otto, A Ivanova, M Herbig, ...

Molecular Metabolism 5 (8), 656-668

112016
Apparatus and method for determining the mechanical properties of cells

J Guck, O Otto, P Rosendahl

US Patent App. 14/913,699

22016
A nanoprinted model of interstitial cancer migration reveals a link between cell deformability and proliferation

M Panagiotakopoulou, M Bergert, A Taubenberger, J Guck, D Poulikakos, ...

ACS Na0 (7), 6437-6448

292016
Real-time deformability cytometry (RT-DC) as novel diagnostic tool in myelodysplastic syndromes

E Bulycheva, O Otto, M Herbig, H Weidner, U Baschant, S Herold, ...

Haematologica 101, 494-495

 2016
3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments

AV Taubenberger, LJ Bray, B Haller, A Shaposhnykov, M Binner, ...

Acta Biomaterialia 36, 73-85

632016
Mechanical phenotyping of leukemic blast cells using real-time deformability cytometry

J Stächele, N Töpfner, C Herold, R Berner, M Bornhäuser, J Guck, ...

Klinische Pädiatrie 228 (03), A2

 2016
Cell nuclei have lower refractive index and mass density than cytoplasm

M Schürmann, J Scholze, P Müller, J Guck, CJ Chan

Journal of Biophotonics 9 (10), 1068-1076

772016
A pH-driven transition of the cytoplasm from a fluid-to a solid-like state promotes entry into dormancy

MC Munder, D Midtvedt, T Franzmann, E Nüske, O Otto, M Herbig, ...

eLife 5, e09347

1402016
Mechanosensing is critical for axon growth in the developing brain

DE Koser, AJ Thompson, SK Foster, A Dwivedy, GK Sheridan, E Pillai, ...

Nature Neuroscience 19 (12), 1592-1598

1742016
Materials and technologies for soft implantable neuroprostheses

S Lacour, G Courtine, J Guck

Nature Reviews Materials 1, 16063

1622016
Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry

M Xavier, P Rosendahl, M Herbig, M Kräter, D Spencer, M Bornhäuser, ...

Integrative Biology 8 (5), 616-623

312016
Refractive index measurements of single, spherical cells using digital holographic microscopy

M Schürmann, J Scholze, P Müller, CJ Chan, AE Ekpenyong, KJ Chalut, ...

Methods in cell biology 125, 143-159

272015
ODTbrain: a Python library for full-view, dense diffraction tomography

P Müller, M Schürmann, J Guck

BMC bioinformatics 16 (1), 367

182015
Mechanotransduction in neutrophil activation and deactivation

AE Ekpenyong, N Toepfner, ER Chilvers, J Guck

Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1853 (11), 3105-3116

222015
Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment

A Mietke, O Otto, S Girardo, P Rosendahl, A Taubenberger, S Golfier, ...

Biophysical Journal 109 (10), 2023-2036

912015
ODTbrain: a Python library for full-view, dense diffraction tomography

P Müller, M Schürmann, J Guck

BMC Bioinformatics 16 (1), 367

182015
Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia.

M Wobus, M Bornhäuser, A Jacobi, M Kräter, O Otto, C Ortlepp, J Guck, ...

Oncotarget 6 (36), 38804-38815

122015
Single-cell diffraction tomography with optofluidic rotation about a tilted axis

P Müller, M Schürmann, CJ Chan, J Guck

Optical Trapping and Optical Micromanipulation XII 9548, 95480U

112015
The Theory of Diffraction Tomography

P Müller, M Schürmann, J Guck

arXiv preprint arXiv:1507.00466

312015
Myosin II Activity Softens Cells in Suspension

CJ Chan, AE Ekpenyong, S Golfier, W Li, KJ Chalut, O Otto, J Elgeti, ...

Biophysical Journal 108 (8), 1856-1869

642015
Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

O Otto, P Rosendahl, A Mietke, S Golfier, C Herold, D Klaue, S Girardo, ...

Nature Methods 12 (3), 199–202

3152015
Deformation of phospholipid vesicles in an optical stretcher

U Delabre, K Feld, E Crespo, G Whyte, C Sykes, U Seifert, J Guck

Soft Matter 11 (30), 6075-6088

262015
A monolithic glass chip for active single-cell sorting based on mechanical phenotyping

C Faigle, F Lautenschläger, G Whyte, P Homewood, E Martín-Badosa, ...

Lab on a Chip 15 (5), 1267-1275

262015
Actin polymerization as a key innate immune effector mechanism to control Salmonella infection

SM Man, A Ekpenyong, P Tourlomousis, S Achouri, E Cammarota, ...

Proceedings of the National Academy of Sciences 111 (49), 17588-17593

612014
Separation of blood cells with differing deformability using deterministic lateral displacement

D Holmes, G Whyte, J Bailey, N Vergara-Irigaray, A Ekpenyong, J Guck, ...

Interface focus 4 (6), 20140011

732014
Dynamic operation of optical fibres beyond the single-mode regime facilitates the orientation of biological cells

M Kreysing, D Ott, MJ Schmidberger, O Otto, M Schürmann, ...

Nature Communications 5, 5481-5486

332014
Direct observation of light focusing by single photoreceptor cell nuclei

Z Błaszczak, M Kreysing, J Guck

Optics Express 22 (9), 11043-11060

152014
The relationship between glial cell mechanosensitivity and foreign body reactions in the central nervous system

P Moshayedi, G Ng, JCF Kwok, GSH Yeo, CE Bryant, JW Fawcett, ...

Biomaterials 35 (13), 3919-3925

1552014
Impact of heating on passive and active biomechanics of suspended cells

CJ Chan, G Whyte, L Boyde, G Salbreux, J Guck

Interface focus 4 (2), 20130069

252014
Grouped retinae and tapetal cups in some Teleostian fish: Occurrence, structure, and function

M Francke, M Kreysing, A Mack, J Engelmann, A Karl, F Makarov, J Guck, ...

Progress in Retinal and Eye Research 38, 43-69

172014
SAMHD1 prevents autoimmunity by maintaining genome stability

S Kretschmer, C Wolf, N König, W Staroske, J Guck, M Häusler, H Luksch, ...

Annals of the rheumatic diseases 74 (3), e17-e17

1012014
Method for diagnosis and/or prognosis of cancers by analysis of the mechanical properties of tumour cells

J Käs, J Guck

US Patent App. 13/877,452

 2013
Mechanics Meets Medicine

J Guck, ER Chilvers

Science translational medicine 5 (212), 212fs41-212fs41

322013
Mechanics in neuronal development and repair

K Franze, PA Janmey, J Guck

Annual review of biomedical engineering 15, 227-251

1662013
Bacterial infection of macrophages induces decrease in refractive index

AE Ekpenyong, SM Man, S Achouri, CE Bryant, J Guck, KJ Chalut

Journal of biophotonics 6 (5), 393-397

372013
Elastic theory for the deformation of a solid or layered spheroid under axisymmetric loading

L Boyde, A Ekpenyong, G Whyte, J Guck

Acta Mechanica 224 (4), 819-839

72013
Cell Sorting

J Guck

Handbook of Biophotonics, 143-149

 2013
Chromatin decondensation and nuclear softening accompany Nanog downregulation in embryonic stem cells

KJ Chalut, M Höpfler, F Lautenschläger, L Boyde, CJ Chan, A Ekpenyong, ...

Biophysical Journal 103 (10), 2060-2070

1082012
Comparison of stresses on homogeneous spheroids in the optical stretcher computed with geometrical optics and generalized Lorenz–Mie theory

L Boyde, A Ekpenyong, G Whyte, J Guck

Applied optics 51 (33), 7934-7944

232012
Femtosecond laser fabricated monolithic devices for single cell manipulation

F Bragheri, P Minzioni, RM Vazquez, N Bellini, P Paiè, C Mondello, ...

Frontiers in Optics, FW1G. 2

12012
Validation and perspectives of a femtosecond laser fabricated monolithic optical stretcher

N Bellini, F Bragheri, I Cristiani, J Guck, R Osellame, G Whyte

Biomedical Optics Express 3 (10), 2658-2668

392012
Viscoelastic properties of differentiating blood cells are fate-and function-dependent

AE Ekpenyong, G Whyte, K Chalut, S Pagliara, F Lautenschläger, ...

PloS one 7 (9), e45237

1232012
Changes in Ect2 localization couple actomyosin-dependent cell shape changes to mitotic progression

HK Matthews, U Delabre, JL Rohn, J Guck, P Kunda, B Baum

Developmental Cell 23 (2), 371-383

1392012
Coupling of active motion and advection shapes intracellular cargo transport

PK Trong, J Guck, RE Goldstein

Physical Review Letters 109 (2), 028104

262012
Photonic crystal light collectors in fish retina improve vision in turbid water

M Kreysing, R Pusch, D Haverkate, M Landsberger, J Engelmann, ...

Science 336 (6089), 1700-1703

552012
Mechanical environment modulates biological properties of oligodendrocyte progenitor cells

A Jagielska, AL Norman, G Whyte, KJV Vliet, J Guck, RJM Franklin

Stem cells and development 21 (16), 2905-2914

752012
Live cells as optical fibers in the vertebrate retina

A Reichenbach, K Franze, S Agte, S Junek, A Wurm, J Grosche, ...

Selected topics on optical fiber technology 10, 247-270

132012
Lebendige Optik

J Guck

Physik Journal 11 (6)

 2012
Quantifying cellular differentiation by physical phenotype using digital holographic microscopy

KJ Chalut, AE Ekpenyong, WL Clegg, IC Melhuish, J Guck

Integrative Biology 4 (3), 280-284

652012
Device and method for the contactless manipulation and alignment of sample particles in a measurement volume using a nonhomogeneous electric alternating field

M Kreysing, J Guck, J Kaes

US Patent 8,076,632

152011
Exact analytical expansion of an off-axis Gaussian laser beam using the translation theorems for the vector spherical harmonics

L Boyde, KJ Chalut, J Guck

Applied optics 50 (7), 1023-1033

92011
Near-and far-field scattering from arbitrary three-dimensional aggregates of coated spheres using parallel computing

L Boyde, KJ Chalut, J Guck

Physical Review E 83 (2), 026701

232011
3D inverted colloidal crystals in realistic cell migration assays for drug screening applications

J da Silva, F Lautenschläger, CHR Kuo, J Guck, E Sivaniah

Integrative Biology 3 (12), 1202-1206

142011
Spatial mapping of the mechanical properties of the living retina using scanning force microscopy

K Franze, M Francke, K Günter, AF Christ, N Körber, A Reichenbach, ...

Soft Matter 7 (7), 3147-3154

752011
Mechanical difference between white and gray matter in the rat cerebellum measured by scanning force microscopy

AF Christ, K Franze, H Gautier, P Moshayedi, J Fawcett, RJM Franklin, ...

Journal of Biomechanics 43 (15), 2986-2992

1772010
Mesenchymal stem cell mechanics from the attached to the suspended state

JM Maloney, D Nikova, F Lautenschläger, E Clarke, R Langer, J Guck, ...

Biophysical Journal 99 (8), 2479-2487

1312010
Micro and nanotechnology for biological and biomedical applications

CT Lim, J Han, J Guck, H Espinosa

Medical & biological engineering & computing 48 (10), 941-943

322010
Biophotonic techniques for the study of malaria-infected red blood cells

JMA Mauritz, A Esposito, T Tiffert, JN Skepper, A Warley, YZ Yoon, ...

Medical & biological engineering & computing 48 (10), 1055-1063

282010
The biophysics of neuronal growth

K Franze, J Guck

Reports on Progress in Physics 73 (9), 094601

1242010
Dual-beam laser traps in biology and medicine: when one beam is not enough

G Whyte, F Lautenschläger, M Kreysing, L Boyde, A Ekpenyong, ...

Optical Trapping and Optical Micromanipulation VII 7762, 77620G

22010
Physical insight into light scattering by photoreceptor cell nuclei

M Kreysing, L Boyde, J Guck, KJ Chalut

Optics Letters 35 (15), 2639-2641

342010
Monitoring of laser micromanipulated optically trapped cells by digital holographic microscopy

B Kemper, P Langehanenberg, A Höink, G von Bally, F Wottowah, ...

Journal of biophotonics 3 (7), 425-431

172010
Mechanosensitivity of astrocytes on optimized polyacrylamide gels analyzed by quantitative morphometry

P Moshayedi, L da F Costa, A Christ, SP Lacour, J Fawcett, J Guck, ...

Journal of Physics: Condensed Matter 22 (19), 194114

962010
Detection of Plasmodium falciparum-infected red blood cells by optical stretching

JMA Mauritz, T Tiffert, R Seear, F Lautenschläger, A Esposito, VL Lew, ...

Journal of biomedical optics 15 (3), 030517-030517-3

392010
The cavity-to-cavity migration of leukaemic cells through 3D honey-combed hydrogels with adjustable internal dimension and stiffness

J da Silva, F Lautenschläger, E Sivaniah, JR Guck

Biomaterials 31 (8), 2201-2208

472010
Critical review: cellular mechanobiology and amoeboid migration

J Guck, F Lautenschläger, S Paschke, M Beil

Integrative Biology 2 (11-12), 575-583

842010
Microfluidic integration of high power dual-beam laser traps for cell mechanical measurements

F Lautenschlaeger, JR Guck

2009 International Symposium on Optomechatronic Technologies, 419-422

32009
The regulatory role of cell mechanics for migration of differentiating myeloid cells

F Lautenschläger, S Paschke, S Schinkinger, A Bruel, M Beil, J Guck

Proceedings of the National Academy of Sciences 106 (37), 15696-15701

1982009
Interaction of Gaussian beam with near-spherical particle: an analytic-numerical approach for assessing scattering and stresses

L Boyde, KJ Chalut, J Guck

JOSA A 26 (8), 1814-1826

372009
Do cells care about physics?

J Guck

Physics World 22 (7), 31-34

32009
Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution

I Solovei, M Kreysing, C Lanctôt, S Kösem, L Peichl, T Cremer, J Guck, ...

Cell 137 (2), 356-368

5862009
Oral cancer diagnosis by mechanical phenotyping

TW Remmerbach, F Wottawah, J Dietrich, B Lincoln, C Wittekind, J Guck

Cancer Research 69 (5), 1728-1732

2802009
Corrigendum to “Quantifying the contribution of actin networks to the elastic strength of fibroblasts”[J. Theor. Biol. 242 (2006) 502–516]

R Ananthakrishnan, J Guck, F Wottawah, S Schinkinger, B Lincoln, ...

Journal of Theoretical Biology 255 (1), 162

12008
The optical cell rotator

MK Kreysing, T Kießling, A Fritsch, C Dietrich, JR Guck, JA Käs

Optics Express 16 (21), 16984-16992

1122008
High‐Throughput Rheological Measurements with an Optical Stretcher

B Lincoln, F Wottawah, S Schinkinger, S Ebert, J Guck

Methods in cell biology 83, 397-423

732007
Fluorescence ratio thermometry in a microfluidic dual-beam laser trap

S Ebert, K Travis, B Lincoln, J Guck

Optics Express 15 (23), 15493-15499

1052007
Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications

B Lincoln, S Schinkinger, K Travis, F Wottawah, S Ebert, F Sauer, J Guck

Biomedical Microdevices 9 (5), 703-710

1372007
Müller cells are living optical fibers in the vertebrate retina

K Franze, J Grosche, SN Skatchkov, S Schinkinger, C Foja, D Schild, ...

Proceedings of the National Academy of Sciences 104 (20), 8287-8292

3552007
Viscoelastic properties of individual glial cells and neurons in the CNS

YB Lu, K Franze, G Seifert, C Steinhäuser, F Kirchhoff, H Wolburg, J Guck, ...

Proceedings of the National Academy of Sciences 103 (47), 17759-17764

4492006
Quantifying the contribution of actin networks to the elastic strength of fibroblasts

R Ananthakrishnan, J Guck, F Wottawah, S Schinkinger, B Lincoln, ...

Journal of theoretical biology 242 (2), 502-516

992006
Scattering from Single Nanoparticles: Mie theory revisited

K Travis, J Guck

Biophysical Reviews and Letters 1 (02), 179-207

22006
The microscopy cell (MicCell), a versatile modular flowthrough system for cell biology, biomaterial research, and nanotechnology

FU Gast, PS Dittrich, P Schwille, M Weigel, M Mertig, J Opitz, U Queitsch, ...

Microfluidics and Nanofluidics 2 (1), 21-36

702006
Excitation beyond the monochromatic laser limit: simultaneous 3-D confocal and multiphoton microscopy with a tapered fiber as white-light laser source

T Betz, J Teipel, D Koch, W Härtig, J Guck, J Käs, H Giessen

Journal of biomedical optics 10 (5), 054009-054009-7

262005
Characterizing single suspended cells by optorheology

F Wottawah, S Schinkinger, B Lincoln, S Ebert, K Müller, F Sauer, K Travis, ...

Acta biomaterialia 1 (3), 263-271

502005
Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence

J Guck, S Schinkinger, B Lincoln, F Wottawah, S Ebert, M Romeyke, ...

Biophysical Journal 88 (5), 3689-3698

11792005
Modelling the structural response of an eukaryotic cell in the optical stretcher

R Ananthakrishnan, J Guck, F Wottawah, S Schinkinger, B Lincoln, ...

Current Science 88 (9)

242005
Optical rheology of biological cells

F Wottawah, S Schinkinger, B Lincoln, R Ananthakrishnan, M Romeyke, ...

Physical review letters 94 (9), 098103

2352005
Feeling for cells with light

S Schinkinger, F Wottawah, K Travis, B Lincoln, J Guck

Optical Trapping and Optical Micromanipulation 5514, 170-178

42004
Deformability‐based flow cytometry

B Lincoln, HM Erickson, S Schinkinger, F Wottawah, D Mitchell, S Ulvick, ...

Cytometry Part A 59 (2), 203-209

1792004
Stretching biological cells with light

J Guck, R Ananthakrishnan, CC Cunningham, J Käs

Journal of Physics: Condensed Matter 14 (19), 4843

1022002
The optical stretcher: a novel laser tool to micromanipulate cells

J Guck, R Ananthakrishnan, H Mahmood, TJ Moon, CC Cunningham, ...

Biophysical Journal 81 (2), 767-784

9222001
Optical deformability: micromechanics from cell research to biomedicine

JR Guck

PhD Thesis

62001
Optical deformability of soft biological dielectrics

J Guck, R Ananthakrishnan, TJ Moon, CC Cunningham, J Käs

Physical Review Letters 84 (23), 5451

3302000
Optical stretcher

JA Kas, JR Guck

US Patent 6,067,859

632000
Dynamics of Single Protein Polymers Visualized by Fluorescence Microscopy

J Käs, J Guck, D Humphrey

Modern Optics, Electronics and High Precision Techniques in Cell Biology …

11998
Optical Stretching–automatisierte Zell-Sortierung und Charakterisierung

S Schinkinger, F Wottawah, B Lincoln, J Guck

LABORWELT, 4



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