Deformability Cytometry Real-Time Deformability Cytometry
- 公司名称 世联博研（北京）科技有限公司
- 品牌 其他品牌
- 型号 Deformability Cytometry
- 产地 德国
- 厂商性质 代理商
- 更新时间 2020/2/21 0:51:48
- 访问次数 987
联系方式：李胜亮18618101725 查看联系方式
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| 产地类别 | 进口 | 产品种类 | 细胞分析系统 |
|---|---|---|---|
| 价格区间 | 面议 | 应用领域 | 医疗卫生,生物产业 |
细胞实时形变测量分析系统，Real-Time Deformability Cytometry
来自德国的高通量单细胞形变测量分析系统
该系统是一套基于微流控流体压力梯度的、在倒置显微镜的扩展起来的、集成流式细胞仪特性、荧光检测模块、温控模 块、高速成像和数据采集分析软件的高通量单细胞实时形变测量和单细胞力学性质分析系统。
是一种以流式细胞仪的速度检测单个细胞形态和力学性质的技术，每个细胞都被实时拍摄、分析和成像存储。 此外，非破坏性的力量应用于细胞，提供一种方便，稳健和高通量的技术进行生物标志物的检测，可用于基础科学和临床研究。
探索细胞的物理特性作为生物标志物,可以将非破坏性的力量应用于细胞或珠子，并观察它们的变形。 这允许研究对物理压力的特定机械响应。
优势亮点：
- 机械力学作为一种新的生物标志物--温和无损伤
- 无标记
- 非破坏性的力量
- 高速测量单个细胞的实时形变（高度、宽度、面积、比例）、高度偏差、环度衡量比、孔度、亮度、杨氏模量等
- 细胞机械特性测量高通量（1000细胞/秒）
- 配有高速成像、荧光检测、温控模块
- 不需要细胞分离/纯化
- 文献量大、级别高文章达数十篇
实时形变
成像
每个细胞被同时拍照、分析和储存。 这允许通过它们的光学特性来找到小亚群或区分细胞。 另外可以研究像表面拓扑或细胞对光的衰减的形态特性。
- 每个获取图像的存储
- 快速访问细胞大小和形态
流式细胞技术
细胞通过微流通道时,提取细胞变形、亮度和大小等参数，同时。 这允许实时地研究细胞属性。
可温控和荧光检测
实时变形细胞计数和同时荧光检测：
- 荧光模块使得该系统不再只是附加了一个额外的细胞力学检测通道的流式细胞仪。它成为了生命科学实验室的得力工具 - 提供了更多视角来解决科学问题。在生物学研究中通常使用荧光流式细胞仪来鉴定和定量细胞和细胞过程。使该系统集荧光流式细胞仪和实时变形的优点于一身，形成了实时荧光形变细胞仪。光片激发设计可实现三通道1D荧光成像。除了所有实时变形参数外，系统还会分析荧光信号实时得到峰图，速度可达每秒1000个细胞。也可在实验后处理保存的原始荧光数据，以针对特定的问题和需 求修改处理方法。
1)根据表面marker鉴定血细胞：
荧光模块可检测和鉴定同一样品中的三种不同荧光。利用标记的表面荧光蛋白可同时实现细胞鉴定和力学性质及形态性质测量。 下图为 G-CSF动员的外周血样品细胞群体。 标记后的细胞表面markers CD3-FITC (T-cells)， CD34-PE (造血干细胞)和CD14-APC(单核细胞)荧光强度检测 揭示了各细胞类型所具有的不同力学性质。- 2)一维荧光成像：
荧光模块在激发光路径中产生一束受限光片，穿过流道，细胞会经过一束很窄的激发光幕。这样可以进行1D荧光成像，例如可用于解析沿流动方向的荧光标记结构的侧向分布。检测到的荧光峰值带有很多重要信息。荧光标记的胞内结构（如细胞核）会显示窄峰，而胞质会显示出更宽的峰。不同分裂期细胞中标记的组蛋白也会呈现出不同的峰图.
加热模块 - 温度控制
加热模块实现了生理温度下的测量。加热模块带有一个300 W的加热器和几个静默通风机来有效混合热空气。靠近样品处有一个传感器和一个控制单元，用以地将温度控制在所需值。系统的空气循环系统非常高效，当进行开放操作（如更换样品）后可以迅速恢复温度。
高速摄像
该成像模块是款高速明场摄像显微镜,使用同步化微秒高强度LED光源减轻运动模糊,可进行极慢运动摄影.每秒可记录500幅全帧图像或10000帧小区域图像
越来越多的研究表明，机械力(Mechanical Forces)是机体生长发育、结构重建以及功能维持的重要因素，也与疾病的发生发展密切相关。力学刺激(Mechanical Stimulation)会对细胞的粘附、铺展、迁移、增殖和分化等行为有着重要的影响，力学表型(Mechanical phenotype)的改变也可用来定量检测、评价细胞的生物学功能。力学生物学(Mechnobiology)研究对深入揭示疾病机理、发展新的诊断治疗方法具有重要意义，还可以为生物材料、再生医学、药物筛选等应用研究提供新思路、新工具。由于细胞内在的生物复杂性及微纳米尺度的制约，如何实现细胞及亚细胞水平上力学信息的实时、原位、动态探测和操控是力学生物学中的挑战性的问题之一。
| Zebrafish spinal cord repair is accompanied by transient tissue stiffening S Möllmert, MA Kharlamova, T Hoche, AV Taubenberger, S Abuhattum, ... Biophysical Journal | 2 | 2019 |
| Polyacrylamide bead sensors for in vivo quantification of cell-scale stress in zebrafish development N Traeber, K Uhlmann, S Girardo, G Kesavan, K Wagner, J Friedrichs, ... Scientific reports 9 (1), 1-14 | 3 | 2019 |
| CASP1 variants influence subcellular caspase-1 localization, pyroptosome formation, pro-inflammatory cell death and macrophage deformability F Kapplusch, F Schulze, S Rabe-Matschewsky, S Russ, M Herbig, ... Clinical Immunology 208, 108232 | 2019 | |
| Morpho‐Rheological Fingerprinting of Rod Photoreceptors Using Real‐Time Deformability Cytometry T Santos‐Ferreira, M Herbig, O Otto, M Carido, MO Karl, S Michalakis, ... Cytometry Part A 95 (11), 1145-1157 | 2019 | |
| 3D microenvironment stiffness regulates tumor spheroid growth and mechanics via p21 and ROCK AV Taubenberger, S Girardo, N Träber, E Fischer‐Friedrich, M Kräter, ... Advanced Biosystems 3 (9), 1900128 | 2 | 2019 |
| Controlling distinct signaling states in cultured cancer cells provides a new platform for drug discovery SW Poser, O Otto, C Arps-Forker, Y Ge, M Herbig, C Andree, ... The FASEB Journal 33 (8), 9235-9249 | 1 | 2019 |
| Erratum: Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation. S Tietze, M Kräter, A Jacobi, A Taubenberger, M Herbig, R Wehner, ... Advanced science (Weinheim, Baden-Wurttemberg, Germany) 6 (8), 1900622-1900622 | 1* | 2019 |
| Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation S Tietze, M Kräter, A Jacobi, A Taubenberger, M Herbig, R Wehner, ... Advanced Science 6 (8), 1802104 | 1 | 2019 |
| Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry A Jacobi, P Rosendahl, M Kräter, M Urbanska, M Herbig, J Guck Stem Cell Mobilization, 135-148 | 2019 | |
| Buckling of epithelium growing under spherical confinement A Trushko, I Di Meglio, A Merzouki, C Blanch-Mercader, S Abuhattum, ... bioRxiv, 513119 | 2 | 2019 |
| Oncogenic signaling alters cell shape and mechanics to facilitate cell division under confinement. HK Matthews, S Ganguli, K Plak, AV Taubenberger, M Piel, J Guck, ... bioRxiv, 571885 | 2019 | |
| The Relationship Between Metastatic Potential and In Vitro Mechanical Properties of Osteosarcoma Cells CN Holenstein, A Horvath, B Schär, AD Schoenenberger, M Bollhalder, ... Molecular Biology of the Cell 30 (7), 887-898 | 2 | 2019 |
| Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming KR Bashant, A Vassallo, C Herold, R Berner, L Menschner, ... Journal of Leukocyte Biology, e12967 | 6 | 2019 |
| Droplet-Assisted Microfluidic Fabrication and Characterization of Multifunctional Polysaccharide Microgels Formed by Multicomponent Reactions N Hauck, N Seixas, S Centeno, R Schlüßler, G Cojoc, P Müller, J Guck, ... Polymers 10 (10), 1055 | 12 | 2018 |
| Mechanical mapping of spinal cord growth and repair in living zebrafish larvae by Brillouin imaging R Schlüßler, S Möllmert, S Abuhattum, G Cojoc, P Müller, K Kim, C Möckel, ... Biophysical journal 115 (5), 911-923 | 31 | 2018 |
| Comparative study of cell mechanics methods PH Wu, DRB Aroush, A Asnacios, WC Chen, ME Dokukin, BL Doss, ... Nature methods 15 (7), 491 | 2018 | |
| A comparison of methods to assess cell mechanical properties PH Wu, DRB Aroush, A Asnacios, WC Chen, ME Dokukin, BL Doss, ... Nature Methods 15 (7), 491-498 | 75 | 2018 |
| Response to Comment on “Cell nuclei have lower refractive index and mass density than cytoplasm” P Müller, J Guck Journal of Biophotonics 11 (6), e201800095 | 1 | 2018 |
| Real-time fluorescence and deformability cytometry P Rosendahl, K Plak, A Jacobi, M Kraeter, N Toepfner, O Otto, C Herold, ... Nature Methods 15 (5), 355-358 | 31 | 2018 |
| Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging P Müller, M Schürmann, S Girardo, G Cojoc, J Guck Optics Express 26 (8), 10729-10743 | 5 | 2018 |
| Toll-like receptor-mediated upregulation of CXCL16 in psoriasis orchestrates neutrophil activation S Steffen, S Abraham, M Herbig, F Schmidt, K Blau, S Meisterfeld, ... Journal of Investigative Dermatology 138 (2), 344-354 | 8 | 2018 |
| Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood N Toepfner, C Herold, O Otto, P Rosendahl, A Jacobi, M Kräter, ... eLife 7, e29213 | 33 | 2018 |
| High-throughput microfluidic characterization of erythrocyte shape and mechanical variability F Reichel, J Mauer, AA Nawaz, G Gompper, J Guck, DA Fedosov bioRxiv, 488189 | 2018 | |
| Alterations in Cell Mechanics by Actin Cytoskeletal Changes Correlate with Strain-Specific Rubella Virus Phenotypes for Cell Migration and Induction of Apoptosis M Kräter, J Sapudom, N Bilz, T Pompe, J Guck, C Claus Cells 7 (9), 136 | 2 | 2018 |
| Acute but not inherited demyelination in mouse models leads to brain tissue stiffness changes D Eberle, G Fodelianaki, T Kurth, A Jagielska, S Moellmert, E Ulbricht, ... bioRxiv, 449603 | 3 | 2018 |
| Axonal transport, phase-separated compartments and neuron mechanics—a new approach to investigate neurodegenerative diseases M Nötzel, G Rosso, S Möllmert, A Seifert, R Schlüßler, K Kim, A Hermann, ... Frontiers in Cellular Neuroscience 12, 358 | 2 | 2018 |
| High-throughput single-cell mechanical phenotyping with real-time deformability cytometry. M Urbanska, P Rosendahl, M Kräter, J Guck Methods in Cell Biology 147, 175-198 | 6 | 2018 |
| Metabolic profiling of human eosinophils L Porter, N Toepfner, KR Bashant, J Guck, M Ashcroft, N Farahi, ... Frontiers in Immunology 9, 1404 | 4 | 2018 |
| Standardized microgel beads as elastic cell mechanical probes S Girardo, N Traeber, K Wagner, G Cojoc, C Herold, R Goswami, ... Journal of Materials Chemistry B | 21 | 2018 |
| Intracellular mass density increase is accompanying but not sufficient for stiffening and growth arrest of yeast cells S Abuhattum, K Kim, TM Franzmann, A Esslinger, D Midtvedt, ... Frontiers in Physics 6, 39-50 | 3 | 2018 |
| Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells M Herbig, M Kräter, K Plak, P Müller, J Guck, O Otto Flow Cytometry Protocols – Methods in Molecular Biology 1678, 347-369 | 19 | 2018 |
| Mechanical mapping of spinal cord development and repair in living zebrafish larvae using Brillouin microscopy R Schlüßler, S Möllmert, S Abuhattum, G Cojoc, P Müller, K Kim, C Möckel, ... bioRxiv, 181560 | 3 | 2018 |
| Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage M Urbanska, M Winzi, K Neumann, S Abuhattum, P Rosendahl, P Müller, ... Development 144 (23), 4313-4321 | 4 | 2017 |
| Three-dimensional correlative single‐cell imaging utilizing fluorescence and refractive index tomography M Schürmann, G Cojoc, S Girardo, E Ulbricht, J Guck, P Müller Journal of Biophotonics 81 (2), e201700145 | 36 | 2017 |
| Method for diagnosis and/or prognosis of cancers by analysis of the mechanical properties of tumor cells J Käs, J Guck US Patent 9,683,982 | 2017 | |
| 3D single-cell imaging using combined fluorescence and refractive index tomography M Schürmann, G Cojoc, S Girardo, E Ulbricht, J Guck, P Müller arXiv preprint arXiv:1706.00715 | 2017 | |
| Mechanical deformation induces depolarization of neutrophils AE Ekpenyong, N Toepfner, C Fiddler, M Herbig, W Li, G Cojoc, ... Science Advances 3 (6), e1602536 | 24 | 2017 |
| Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells S Tavares, AF Vieira, AV Taubenberger, M Araújo, NP Martins, ... Nature Communications 8, 15237 | 45 | 2017 |
| Mechanical strain promotes oligodendrocyte differentiation by global changes of gene expression A Jagielska, AL Lowe, E Makhija, L Wroblewska, J Guck, RJM Franklin, ... Frontiers in cellular neuroscience 11, 93 | 25 | 2017 |
| Initiation of acute graft-versus-host disease by angiogenesis K Riesner, Y Shi, A Jacobi, M Kraeter, M Kalupa, A McGearey, S Mertlitz, ... Blood 129 (14), 2021-2032 | 23 | 2017 |
| Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion M Koch, KE Wright, O Otto, M Herbig, ND Salinas, NH Tolia, TJ Satchwell, ... Proceedings of the National Academy of Sciences 114 (16), 4225-4230 | 26 | 2017 |
| High‐throughput cell mechanical phenotyping for label‐free titration assays of cytoskeletal modifications S Golfier, P Rosendahl, A Mietke, M Herbig, J Guck, O Otto Cytoskeleton 74 (8), 283-296 | 21 | 2017 |
| Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase CJ Chan, W Li, G Cojoc, J Guck Biophysical Journal 112 (6), 1063-1076 | 10 | 2017 |
| V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling-a new option for HCC therapy K Bartel, M Winzi, M Ulrich, A Koeberle, D Menche, O Werz, R Müller, ... Oncotarget 8 (6), 9476 | 17 | 2017 |
| Numerical simulation of real-time deformability cytometry to extract cell mechanical properties M Mokbel, D Mokbel, A Mietke, N Traber, S Girardo, O Otto, J Guck, ... ACS Biomaterials Science & Engineering 3 (11), 2962-2973 | 47 | 2017 |
| Real-time fluorescence and deformability cytometry-flow cytometry goes mechanics P Rosendahl, K Plak, A Jacobi, M Kraeter, N Toepfner, O Otto, C Herold, ... bioRxiv, 187435 | 2 | 2017 |
| Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion A Surcel, ES Schiffhauer, D Thomas, Q Zhu, K DiNapoli, M Herbig, O Otto, ... bioRxiv, 190553 | 5 | 2017 |
| Roadmap on optofluidics P Minzioni, R Osellame, C Sada, S Zhao, F Omenetto, K Gylfason, ... Journal of Optics 19 (9), 093003 | 30 | 2017 |
| Bone marrow niche-mimetics modulate HSPC function via integrin signaling M Kräter, A Jacobi, O Otto, S Tietze, K Müller, DM Poitz, S Palm, VM Zinna, ... Scientific Reports 7 (1), 2549 | 12 | 2017 |
| Detection Of Human Disease Conditions By Single-Cell Morpho-Rheological Phenotyping Of Whole Blood N Toepfner, C Herold, O Otto, P Rosendahl, A Jacobi, M Krater, ... bioRxiv, 145078 | 3 | 2017 |
| Mechanical mismatch between Ras transformed and untransformed epithelial cells C Gullekson, G Cojoc, M Schürmann, J Guck, A Pelling Soft Matter 13 (45), 8483-8491 | 3 | 2017 |
| Enlightening discriminative network functional modules behind Principal Component Analysis separation in differential-omic science studies S Ciucci, Y Ge, C Durán, A Palladini, V Jiménez-Jiménez, ... Scientific Reports 7, 43946 | 26 | 2017 |
| Niche WNT5A regulates the actin cytoskeleton during regeneration of hematopoietic stem cells C Schreck, R Istvánffy, C Ziegenhain, T Sippenauer, F Ruf, L Henkel, ... Journal of Experimental Medicine 214 (1), 156-181 | 13 | 2016 |
| Initiation of Acute Graft-Versus-Host Disease By Angiogenesis K Riesner, Y Shi, A Jacobi, M Kraeter, M Kalupa, A McGearey, S Mertlitz, ... Blood, The Journal of the American Society of Hematology 128 (22), 4533-4533 | 2016 | |
| Chemotherapy impedes in vitro microcirculation and promotes migration of leukemic cells with impact on metastasis SV Prathivadhi-Bhayankaram, J Ning, M Mimlitz, C Taylor, E Gross, ... Biochemical and Biophysical Research Communications 479 (4), 841-846 | 5 | 2016 |
| Brain tissue stiffness is a sensitive marker for acidosis K Holtzmann, HOB Gautier, AF Christ, J Guck, RT Káradóttir, K Franze Journal of Neuroscience Methods 271, 50-54 | 17 | 2016 |
| The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512 H Mziaut, B Mulligan, P Hoboth, O Otto, A Ivanova, M Herbig, ... Molecular Metabolism 5 (8), 656-668 | 11 | 2016 |
| Apparatus and method for determining the mechanical properties of cells J Guck, O Otto, P Rosendahl US Patent App. 14/913,699 | 2 | 2016 |
| A nanoprinted model of interstitial cancer migration reveals a link between cell deformability and proliferation M Panagiotakopoulou, M Bergert, A Taubenberger, J Guck, D Poulikakos, ... ACS Na0 (7), 6437-6448 | 29 | 2016 |
| Real-time deformability cytometry (RT-DC) as novel diagnostic tool in myelodysplastic syndromes E Bulycheva, O Otto, M Herbig, H Weidner, U Baschant, S Herold, ... Haematologica 101, 494-495 | 2016 | |
| 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments AV Taubenberger, LJ Bray, B Haller, A Shaposhnykov, M Binner, ... Acta Biomaterialia 36, 73-85 | 63 | 2016 |
| Mechanical phenotyping of leukemic blast cells using real-time deformability cytometry J Stächele, N Töpfner, C Herold, R Berner, M Bornhäuser, J Guck, ... Klinische Pädiatrie 228 (03), A2 | 2016 | |
| Cell nuclei have lower refractive index and mass density than cytoplasm M Schürmann, J Scholze, P Müller, J Guck, CJ Chan Journal of Biophotonics 9 (10), 1068-1076 | 77 | 2016 |
| A pH-driven transition of the cytoplasm from a fluid-to a solid-like state promotes entry into dormancy MC Munder, D Midtvedt, T Franzmann, E Nüske, O Otto, M Herbig, ... eLife 5, e09347 | 140 | 2016 |
| Mechanosensing is critical for axon growth in the developing brain DE Koser, AJ Thompson, SK Foster, A Dwivedy, GK Sheridan, E Pillai, ... Nature Neuroscience 19 (12), 1592-1598 | 174 | 2016 |
| Materials and technologies for soft implantable neuroprostheses S Lacour, G Courtine, J Guck Nature Reviews Materials 1, 16063 | 162 | 2016 |
| Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry M Xavier, P Rosendahl, M Herbig, M Kräter, D Spencer, M Bornhäuser, ... Integrative Biology 8 (5), 616-623 | 31 | 2016 |
| Refractive index measurements of single, spherical cells using digital holographic microscopy M Schürmann, J Scholze, P Müller, CJ Chan, AE Ekpenyong, KJ Chalut, ... Methods in cell biology 125, 143-159 | 27 | 2015 |
| ODTbrain: a Python library for full-view, dense diffraction tomography P Müller, M Schürmann, J Guck BMC bioinformatics 16 (1), 367 | 18 | 2015 |
| Mechanotransduction in neutrophil activation and deactivation AE Ekpenyong, N Toepfner, ER Chilvers, J Guck Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1853 (11), 3105-3116 | 22 | 2015 |
| Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment A Mietke, O Otto, S Girardo, P Rosendahl, A Taubenberger, S Golfier, ... Biophysical Journal 109 (10), 2023-2036 | 91 | 2015 |
| ODTbrain: a Python library for full-view, dense diffraction tomography P Müller, M Schürmann, J Guck BMC Bioinformatics 16 (1), 367 | 18 | 2015 |
| Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia. M Wobus, M Bornhäuser, A Jacobi, M Kräter, O Otto, C Ortlepp, J Guck, ... Oncotarget 6 (36), 38804-38815 | 12 | 2015 |
| Single-cell diffraction tomography with optofluidic rotation about a tilted axis P Müller, M Schürmann, CJ Chan, J Guck Optical Trapping and Optical Micromanipulation XII 9548, 95480U | 11 | 2015 |
| The Theory of Diffraction Tomography P Müller, M Schürmann, J Guck arXiv preprint arXiv:1507.00466 | 31 | 2015 |
| Myosin II Activity Softens Cells in Suspension CJ Chan, AE Ekpenyong, S Golfier, W Li, KJ Chalut, O Otto, J Elgeti, ... Biophysical Journal 108 (8), 1856-1869 | 64 | 2015 |
| Real-time deformability cytometry: on-the-fly cell mechanical phenotyping O Otto, P Rosendahl, A Mietke, S Golfier, C Herold, D Klaue, S Girardo, ... Nature Methods 12 (3), 199–202 | 315 | 2015 |
| Deformation of phospholipid vesicles in an optical stretcher U Delabre, K Feld, E Crespo, G Whyte, C Sykes, U Seifert, J Guck Soft Matter 11 (30), 6075-6088 | 26 | 2015 |
| A monolithic glass chip for active single-cell sorting based on mechanical phenotyping C Faigle, F Lautenschläger, G Whyte, P Homewood, E Martín-Badosa, ... Lab on a Chip 15 (5), 1267-1275 | 26 | 2015 |
| Actin polymerization as a key innate immune effector mechanism to control Salmonella infection SM Man, A Ekpenyong, P Tourlomousis, S Achouri, E Cammarota, ... Proceedings of the National Academy of Sciences 111 (49), 17588-17593 | 61 | 2014 |
| Separation of blood cells with differing deformability using deterministic lateral displacement D Holmes, G Whyte, J Bailey, N Vergara-Irigaray, A Ekpenyong, J Guck, ... Interface focus 4 (6), 20140011 | 73 | 2014 |
| Dynamic operation of optical fibres beyond the single-mode regime facilitates the orientation of biological cells M Kreysing, D Ott, MJ Schmidberger, O Otto, M Schürmann, ... Nature Communications 5, 5481-5486 | 33 | 2014 |
| Direct observation of light focusing by single photoreceptor cell nuclei Z Błaszczak, M Kreysing, J Guck Optics Express 22 (9), 11043-11060 | 15 | 2014 |
| The relationship between glial cell mechanosensitivity and foreign body reactions in the central nervous system P Moshayedi, G Ng, JCF Kwok, GSH Yeo, CE Bryant, JW Fawcett, ... Biomaterials 35 (13), 3919-3925 | 155 | 2014 |
| Impact of heating on passive and active biomechanics of suspended cells CJ Chan, G Whyte, L Boyde, G Salbreux, J Guck Interface focus 4 (2), 20130069 | 25 | 2014 |
| Grouped retinae and tapetal cups in some Teleostian fish: Occurrence, structure, and function M Francke, M Kreysing, A Mack, J Engelmann, A Karl, F Makarov, J Guck, ... Progress in Retinal and Eye Research 38, 43-69 | 17 | 2014 |
| SAMHD1 prevents autoimmunity by maintaining genome stability S Kretschmer, C Wolf, N König, W Staroske, J Guck, M Häusler, H Luksch, ... Annals of the rheumatic diseases 74 (3), e17-e17 | 101 | 2014 |
| Method for diagnosis and/or prognosis of cancers by analysis of the mechanical properties of tumour cells J Käs, J Guck US Patent App. 13/877,452 | 2013 | |
| Mechanics Meets Medicine J Guck, ER Chilvers Science translational medicine 5 (212), 212fs41-212fs41 | 32 | 2013 |
| Mechanics in neuronal development and repair K Franze, PA Janmey, J Guck Annual review of biomedical engineering 15, 227-251 | 166 | 2013 |
| Bacterial infection of macrophages induces decrease in refractive index AE Ekpenyong, SM Man, S Achouri, CE Bryant, J Guck, KJ Chalut Journal of biophotonics 6 (5), 393-397 | 37 | 2013 |
| Elastic theory for the deformation of a solid or layered spheroid under axisymmetric loading L Boyde, A Ekpenyong, G Whyte, J Guck Acta Mechanica 224 (4), 819-839 | 7 | 2013 |
| Cell Sorting J Guck Handbook of Biophotonics, 143-149 | 2013 | |
| Chromatin decondensation and nuclear softening accompany Nanog downregulation in embryonic stem cells KJ Chalut, M Höpfler, F Lautenschläger, L Boyde, CJ Chan, A Ekpenyong, ... Biophysical Journal 103 (10), 2060-2070 | 108 | 2012 |
| Comparison of stresses on homogeneous spheroids in the optical stretcher computed with geometrical optics and generalized Lorenz–Mie theory L Boyde, A Ekpenyong, G Whyte, J Guck Applied optics 51 (33), 7934-7944 | 23 | 2012 |
| Femtosecond laser fabricated monolithic devices for single cell manipulation F Bragheri, P Minzioni, RM Vazquez, N Bellini, P Paiè, C Mondello, ... Frontiers in Optics, FW1G. 2 | 1 | 2012 |
| Validation and perspectives of a femtosecond laser fabricated monolithic optical stretcher N Bellini, F Bragheri, I Cristiani, J Guck, R Osellame, G Whyte Biomedical Optics Express 3 (10), 2658-2668 | 39 | 2012 |
| Viscoelastic properties of differentiating blood cells are fate-and function-dependent AE Ekpenyong, G Whyte, K Chalut, S Pagliara, F Lautenschläger, ... PloS one 7 (9), e45237 | 123 | 2012 |
| Changes in Ect2 localization couple actomyosin-dependent cell shape changes to mitotic progression HK Matthews, U Delabre, JL Rohn, J Guck, P Kunda, B Baum Developmental Cell 23 (2), 371-383 | 139 | 2012 |
| Coupling of active motion and advection shapes intracellular cargo transport PK Trong, J Guck, RE Goldstein Physical Review Letters 109 (2), 028104 | 26 | 2012 |
| Photonic crystal light collectors in fish retina improve vision in turbid water M Kreysing, R Pusch, D Haverkate, M Landsberger, J Engelmann, ... Science 336 (6089), 1700-1703 | 55 | 2012 |
| Mechanical environment modulates biological properties of oligodendrocyte progenitor cells A Jagielska, AL Norman, G Whyte, KJV Vliet, J Guck, RJM Franklin Stem cells and development 21 (16), 2905-2914 | 75 | 2012 |
| Live cells as optical fibers in the vertebrate retina A Reichenbach, K Franze, S Agte, S Junek, A Wurm, J Grosche, ... Selected topics on optical fiber technology 10, 247-270 | 13 | 2012 |
| Lebendige Optik J Guck Physik Journal 11 (6) | 2012 | |
| Quantifying cellular differentiation by physical phenotype using digital holographic microscopy KJ Chalut, AE Ekpenyong, WL Clegg, IC Melhuish, J Guck Integrative Biology 4 (3), 280-284 | 65 | 2012 |
| Device and method for the contactless manipulation and alignment of sample particles in a measurement volume using a nonhomogeneous electric alternating field M Kreysing, J Guck, J Kaes US Patent 8,076,632 | 15 | 2011 |
| Exact analytical expansion of an off-axis Gaussian laser beam using the translation theorems for the vector spherical harmonics L Boyde, KJ Chalut, J Guck Applied optics 50 (7), 1023-1033 | 9 | 2011 |
| Near-and far-field scattering from arbitrary three-dimensional aggregates of coated spheres using parallel computing L Boyde, KJ Chalut, J Guck Physical Review E 83 (2), 026701 | 23 | 2011 |
| 3D inverted colloidal crystals in realistic cell migration assays for drug screening applications J da Silva, F Lautenschläger, CHR Kuo, J Guck, E Sivaniah Integrative Biology 3 (12), 1202-1206 | 14 | 2011 |
| Spatial mapping of the mechanical properties of the living retina using scanning force microscopy K Franze, M Francke, K Günter, AF Christ, N Körber, A Reichenbach, ... Soft Matter 7 (7), 3147-3154 | 75 | 2011 |
| Mechanical difference between white and gray matter in the rat cerebellum measured by scanning force microscopy AF Christ, K Franze, H Gautier, P Moshayedi, J Fawcett, RJM Franklin, ... Journal of Biomechanics 43 (15), 2986-2992 | 177 | 2010 |
| Mesenchymal stem cell mechanics from the attached to the suspended state JM Maloney, D Nikova, F Lautenschläger, E Clarke, R Langer, J Guck, ... Biophysical Journal 99 (8), 2479-2487 | 131 | 2010 |
| Micro and nanotechnology for biological and biomedical applications CT Lim, J Han, J Guck, H Espinosa Medical & biological engineering & computing 48 (10), 941-943 | 32 | 2010 |
| Biophotonic techniques for the study of malaria-infected red blood cells JMA Mauritz, A Esposito, T Tiffert, JN Skepper, A Warley, YZ Yoon, ... Medical & biological engineering & computing 48 (10), 1055-1063 | 28 | 2010 |
| The biophysics of neuronal growth K Franze, J Guck Reports on Progress in Physics 73 (9), 094601 | 124 | 2010 |
| Dual-beam laser traps in biology and medicine: when one beam is not enough G Whyte, F Lautenschläger, M Kreysing, L Boyde, A Ekpenyong, ... Optical Trapping and Optical Micromanipulation VII 7762, 77620G | 2 | 2010 |
| Physical insight into light scattering by photoreceptor cell nuclei M Kreysing, L Boyde, J Guck, KJ Chalut Optics Letters 35 (15), 2639-2641 | 34 | 2010 |
| Monitoring of laser micromanipulated optically trapped cells by digital holographic microscopy B Kemper, P Langehanenberg, A Höink, G von Bally, F Wottowah, ... Journal of biophotonics 3 (7), 425-431 | 17 | 2010 |
| Mechanosensitivity of astrocytes on optimized polyacrylamide gels analyzed by quantitative morphometry P Moshayedi, L da F Costa, A Christ, SP Lacour, J Fawcett, J Guck, ... Journal of Physics: Condensed Matter 22 (19), 194114 | 96 | 2010 |
| Detection of Plasmodium falciparum-infected red blood cells by optical stretching JMA Mauritz, T Tiffert, R Seear, F Lautenschläger, A Esposito, VL Lew, ... Journal of biomedical optics 15 (3), 030517-030517-3 | 39 | 2010 |
| The cavity-to-cavity migration of leukaemic cells through 3D honey-combed hydrogels with adjustable internal dimension and stiffness J da Silva, F Lautenschläger, E Sivaniah, JR Guck Biomaterials 31 (8), 2201-2208 | 47 | 2010 |
| Critical review: cellular mechanobiology and amoeboid migration J Guck, F Lautenschläger, S Paschke, M Beil Integrative Biology 2 (11-12), 575-583 | 84 | 2010 |
| Microfluidic integration of high power dual-beam laser traps for cell mechanical measurements F Lautenschlaeger, JR Guck 2009 International Symposium on Optomechatronic Technologies, 419-422 | 3 | 2009 |
| The regulatory role of cell mechanics for migration of differentiating myeloid cells F Lautenschläger, S Paschke, S Schinkinger, A Bruel, M Beil, J Guck Proceedings of the National Academy of Sciences 106 (37), 15696-15701 | 198 | 2009 |
| Interaction of Gaussian beam with near-spherical particle: an analytic-numerical approach for assessing scattering and stresses L Boyde, KJ Chalut, J Guck JOSA A 26 (8), 1814-1826 | 37 | 2009 |
| Do cells care about physics? J Guck Physics World 22 (7), 31-34 | 3 | 2009 |
| Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution I Solovei, M Kreysing, C Lanctôt, S Kösem, L Peichl, T Cremer, J Guck, ... Cell 137 (2), 356-368 | 586 | 2009 |
| Oral cancer diagnosis by mechanical phenotyping TW Remmerbach, F Wottawah, J Dietrich, B Lincoln, C Wittekind, J Guck Cancer Research 69 (5), 1728-1732 | 280 | 2009 |
| Corrigendum to “Quantifying the contribution of actin networks to the elastic strength of fibroblasts”[J. Theor. Biol. 242 (2006) 502–516] R Ananthakrishnan, J Guck, F Wottawah, S Schinkinger, B Lincoln, ... Journal of Theoretical Biology 255 (1), 162 | 1 | 2008 |
| The optical cell rotator MK Kreysing, T Kießling, A Fritsch, C Dietrich, JR Guck, JA Käs Optics Express 16 (21), 16984-16992 | 112 | 2008 |
| High‐Throughput Rheological Measurements with an Optical Stretcher B Lincoln, F Wottawah, S Schinkinger, S Ebert, J Guck Methods in cell biology 83, 397-423 | 73 | 2007 |
| Fluorescence ratio thermometry in a microfluidic dual-beam laser trap S Ebert, K Travis, B Lincoln, J Guck Optics Express 15 (23), 15493-15499 | 105 | 2007 |
| Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications B Lincoln, S Schinkinger, K Travis, F Wottawah, S Ebert, F Sauer, J Guck Biomedical Microdevices 9 (5), 703-710 | 137 | 2007 |
| Müller cells are living optical fibers in the vertebrate retina K Franze, J Grosche, SN Skatchkov, S Schinkinger, C Foja, D Schild, ... Proceedings of the National Academy of Sciences 104 (20), 8287-8292 | 355 | 2007 |
| Viscoelastic properties of individual glial cells and neurons in the CNS YB Lu, K Franze, G Seifert, C Steinhäuser, F Kirchhoff, H Wolburg, J Guck, ... Proceedings of the National Academy of Sciences 103 (47), 17759-17764 | 449 | 2006 |
| Quantifying the contribution of actin networks to the elastic strength of fibroblasts R Ananthakrishnan, J Guck, F Wottawah, S Schinkinger, B Lincoln, ... Journal of theoretical biology 242 (2), 502-516 | 99 | 2006 |
| Scattering from Single Nanoparticles: Mie theory revisited K Travis, J Guck Biophysical Reviews and Letters 1 (02), 179-207 | 2 | 2006 |
| The microscopy cell (MicCell), a versatile modular flowthrough system for cell biology, biomaterial research, and nanotechnology FU Gast, PS Dittrich, P Schwille, M Weigel, M Mertig, J Opitz, U Queitsch, ... Microfluidics and Nanofluidics 2 (1), 21-36 | 70 | 2006 |
| Excitation beyond the monochromatic laser limit: simultaneous 3-D confocal and multiphoton microscopy with a tapered fiber as white-light laser source T Betz, J Teipel, D Koch, W Härtig, J Guck, J Käs, H Giessen Journal of biomedical optics 10 (5), 054009-054009-7 | 26 | 2005 |
| Characterizing single suspended cells by optorheology F Wottawah, S Schinkinger, B Lincoln, S Ebert, K Müller, F Sauer, K Travis, ... Acta biomaterialia 1 (3), 263-271 | 50 | 2005 |
| Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence J Guck, S Schinkinger, B Lincoln, F Wottawah, S Ebert, M Romeyke, ... Biophysical Journal 88 (5), 3689-3698 | 1179 | 2005 |
| Modelling the structural response of an eukaryotic cell in the optical stretcher R Ananthakrishnan, J Guck, F Wottawah, S Schinkinger, B Lincoln, ... Current Science 88 (9) | 24 | 2005 |
| Optical rheology of biological cells F Wottawah, S Schinkinger, B Lincoln, R Ananthakrishnan, M Romeyke, ... Physical review letters 94 (9), 098103 | 235 | 2005 |
| Feeling for cells with light S Schinkinger, F Wottawah, K Travis, B Lincoln, J Guck Optical Trapping and Optical Micromanipulation 5514, 170-178 | 4 | 2004 |
| Deformability‐based flow cytometry B Lincoln, HM Erickson, S Schinkinger, F Wottawah, D Mitchell, S Ulvick, ... Cytometry Part A 59 (2), 203-209 | 179 | 2004 |
| Stretching biological cells with light J Guck, R Ananthakrishnan, CC Cunningham, J Käs Journal of Physics: Condensed Matter 14 (19), 4843 | 102 | 2002 |
| The optical stretcher: a novel laser tool to micromanipulate cells J Guck, R Ananthakrishnan, H Mahmood, TJ Moon, CC Cunningham, ... Biophysical Journal 81 (2), 767-784 | 922 | 2001 |
| Optical deformability: micromechanics from cell research to biomedicine JR Guck PhD Thesis | 6 | 2001 |
| Optical deformability of soft biological dielectrics J Guck, R Ananthakrishnan, TJ Moon, CC Cunningham, J Käs Physical Review Letters 84 (23), 5451 | 330 | 2000 |
| Optical stretcher JA Kas, JR Guck US Patent 6,067,859 | 63 | 2000 |
| Dynamics of Single Protein Polymers Visualized by Fluorescence Microscopy J Käs, J Guck, D Humphrey Modern Optics, Electronics and High Precision Techniques in Cell Biology … | 1 | 1998 |
| Optical Stretching–automatisierte Zell-Sortierung und Charakterisierung S Schinkinger, F Wottawah, B Lincoln, J Guck LABORWELT, 4 |
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