T9100Technovit9100树脂包埋试剂盒

Technovit9100树脂包埋试剂盒

Newly introduced! Technovit® 9100 is an embedding medium system based on methyl methacrylate （MMA）, which hardens at low temperature. It is designed for the embedding of mineralized tissues with extensive possibilities of staining for light microscopy.

Applications:

• Heavy duty microtome technique for preparing thin sections – such as pelvic biopsy, small low and high density bone samples.
• Cutting grinding and micro-grinding system （contact point technique） - such as jaw and teeth segments with and without implant.
• Combined contact point and heavy duty microtome technique （target preparation） - such as interface regions and examination of tissues surrounding metal implants and cement-free endoprothesis （target tissue preparation）.

Properties:

Technovit® 9100 Polymerization takes place under the exclusion of oxygen with the help of a peroxide/amine catalyst. Additional components such as PMMA-powder and a regulator allows for a controlled polymerization at temperatures between –8 and –20°C, which guarantees for the dispersion of the heat generated during polymerization. The polymerization time is between 18 and 24 hours using a volume between 3 and 15 ml in the above temperature range. All the routine techniques such as preparation tissues, staining, immuno- and enzyme-histochemistry, in-situ hybridization techniques can be performed.

• Basic Solution – is composed of organic monomers, with at least one carbon-carbon double bond （C=C）. The stabilizer added to this solution is for storage-stability. Hydrophilic properties are improved by the addition of a special hydrophilic generating agent.
• PMMA-Powder – is an internal filler and it is made up of PMMAmicro pellets. It is used to:
  - Reduce the polymerization shrinking effect.
  - Reduce the heat generated during the polymerization process. 
  - Improve the quality of the polymerization block.

• Hardener 1 – is one of the components of the polymerization initiation system. It is a derivative of dibenzoyl peroxide, which in combination with Hardener 2, makes the polymerization take place.
• Hardener 2 – it works catalytically upon hardener 1 to allows for a controlled polymerization, even at temperatures below 0°C.
• Regulator – is composed of a reactive organic compound, which allows a controlled polymerization, even with large volumes of polymer, without increases in the temperature during the polymerization reaction.
• PMMA-Granulate, EXART® - this granulate acts as an additional internal filler when larger amounts （500-1000 ml） of polymer are to be used, for example, in the case of femur shaft with noncemented endoprosthses. The amount of monomer （basic solution） is thereby reduced, at the same time making the polymerization easier to control.

Technovit9100树脂包埋试剂盒

Technovit®9100聚合反应是在过氧化物/胺催化剂帮助下排除氧气的情况下进行的。其他组分如PMMA-粉末和调节剂允许在-8和-20°C之间的温度下进行受控聚合，这保证了聚合过程中产生的热量的分散。在上述温度范围内，使用3至15ml体积的聚合时间为18至24小时。所有的常规技术，如准备组织，染色，免疫组化和酶组织化学，原位杂交技术都可以进行。
碱性溶液 - 由有机单体组成，至少有一个碳 - 碳双键（C = C）。添加到该溶液中的稳定剂用于储存稳定性。通过添加特殊的亲水性发生剂来改善亲水性。
PMMA-粉末 - 是一种内部填充物，由PMMAmicro颗粒组成。它用于：
- 减少聚合收缩效应。
- 减少聚合过程中产生的热量。
- 改善聚合块的质量。
硬化剂1 - 是聚合引发体系的组分之一。它是过氧化二苯甲酰的衍生物，与硬化剂2结合使用，可以发生聚合反应。
固化剂2 - 它在固化剂1上起催化作用，即使在低于0°C的温度下也可进行受控聚合。
调节器 - 由反应性有机化合物组成，即使在大量聚合物的情况下也可以进行受控聚合反应，而不会在聚合反应过程中升高温度。
PMMA-颗粒，EXART®--当需要使用大量（500-1000毫升）聚合物时，这种颗粒作为额外的内部填充剂，例如，对于非内源性endoprosthses股骨干的情况下。由此降低单体（碱性溶液）的量，同时使聚合更容易控制。