7076S-CST Anti-mouse IgG, HRP-linked Antibody_CST-上海盈东生物科技有限公司

CST Anti-mouse IgG, HRP-linked Antibody 返回列表页

参考价: ￥ 1500

订货量: ≥1 件

具体成交价以合同协议为准

产品型号7076S

品牌CST

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更新时间：2023-12-19 19:33:51浏览次数：678次

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供货周期	一周	规格	1ml
货号	7076S

供货周期

一周

规格

1ml

货号

7076S

Product Usage Information

Recommended Antibody Dilutions:

1:1000–1:3000

20X LumiGLO® Reagent and 20X Peroxide #7003 1:1K–1:3K

SignalFireTM ECL Reagent #6883 1:1K–1:3K

SignalFireTM Plus ECL Reagent #12630 1:5K-1:15K

SignalFireTM Elite ECL Reagent #12757 1:10K-1:20K

Storage

Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at –20°C. Do not aliquot the antibodies. This product is stable for 60 months when stored at -20C.

Product Description

Affinity purified horse anti-mouse IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection. This product is thoroughly validated with CST primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results.

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

仅限科研使用

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

Source/Isotype:

Horse