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| 供货周期 | 一个月 |
|---|
产品简介
详细介绍
产品详情：
Cube Biotech公司的GST/Gluthathione亲和琼脂糖已成功发表在以下出版物：
PureCube Glutathione磁珠的效率:
图1：可靠的批次间再现性。GST从大肠杆菌裂解物中纯化，利用三个独立生产不同批次的PureCube Glutathione磁珠。FT：穿流，E1-E5：洗脱组分。我们的GST标签纯化产品能够复制所有的纯化试验从批次到批次。产品在多次使用后实现了尽可能小的波动，并且多次连续的GST标签纯化在使用相同的磁珠的情况下能确保相同的高质量。
产品参数：
应用 | Specific binding and purification of GST-tagged proteins |
特异性 | Affinity to GST-tagged proteins |
结合能力 | 10 mg / ml |
Bead Ligand | Protein purification beads with GST-Antibody |
粒径大小 | 40 μm |
pH稳定性 | 3-12 |
推荐流速 | 0.25-1.0 mL/min |
推荐操作压力 | <5bar (72 psi) |
保存温度 | 2℃到8℃ |
订购详情：
货号 | 产品 | 粒径 （μm） | 每毫升磁 珠载量 | 运输 温度 | 储存 温度 | 应用 |
32101 | PureCube Glutathione Agarose(1mL) | 40 | 10mg/mL | 室温 | 4° C | 用于纯化含GST的重组融合蛋白或依赖转 S- 移酶或Glutathione的蛋白 ,pH 稳定工作范围3-12, 最大流速为6mL/min |
32103 | PureCube Glutathione Agarose(10mL) | 40 | 10mg/mL | 室温 | 4° C | |
32105 | PureCube Glutathione Agarose(50mL) | 40 | 10mg/mL | 室温 | 4° C | |
32110 | PureCube Glutathione Agarose(250mL) | 40 | 10mg/mL | 室温 | 4° C | |
32112 | PureCube Glutathione Agarose(500mL) | 40 | 10mg/mL | 室温 | 4° C | |
32115 | PureCube Glutathione Agarose(1000mL) | 40 | 10mg/mL | 室温 | 4° C | |
32120 | PureCube Glutathione Agarose(5000mL) | 40 | 10mg/mL | 室温 | 4° C |
文献参考：
1.Kilkenny, M. L et al. “The human CTF4-orthologue AND-1 interacts with DNA polymerase α/primase via its unique C-terminal HMG box." Open biology vol. 7,11 (2017): 170217. doi:10.1098/rsob.170217.
2. Napolitano, Anna et al. “Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+ Dendritic Cells at the Site of Infection." Journal of immunology (Baltimore, Md. : 1950) vol. 201,2 (2018): 604-614. doi:10.4049/jimmunol.1701322
3. Boor, Susanne de (2015). Mechanistic studies on the regulation of Ran-function by post-translational lysine-acetylation. Dissertation, Universität zu Köln.
4. Fischer K. (2015). TBX3-Mutationsanalysen und Konstruktion einer Zelllinie für TBX2-Inhibitorscreenings. Dissertation, Universität zu Mainz.
5. A. Sommerkamp, et al. (2019). Crystal structure of the first eukaryotic bilin reductase Gt PEBB reveals a flipped binding mode of dihydrobiliverdin. Journal of Biological Chemistry. jbc.RA119.009306. 10.1074/jbc.RA119.009306.
6. Chiocchini C., Trefzer A., Vattem K., Kuhn P., inventor; Thermo Fisher Scientific Geneart GmBH, Pierce Biotechnology Inc, assignee. Devices and methods for producing nucleic acids and proteins. United States patent US20180291413A1. 2016 April 10.
7. Chiocchini, C. et al. (2020). From Electronic Sequence to Purified Protein Using Automated Gene Synthesis and In Vitro Transcription/Translation. ACS Synthetic Biology. XXXX. 10.1021/acssynbio.0c00060.