thermo Qubit ssDNA Assay Kit

thermo Qubit ssDNA Assay Kit

Qubit荧光计工作原理
单个样品检测非常简单
便于使用的荧光计，可快速简单地获得实验结果 大尺寸的彩色触摸屏，可轻松进行各种不同操作。 计算和设置都由仪器自动完成 (图 1)。 Qubit 4荧光计可保存高达1000个样品的数据，数据可由USB数据线或U盘传输。 此外，新的Qubit 4还包含一个内置的试剂计算器，可以轻松确定准备样品和标准所需的染料和缓冲液的量 （图2）。

适用于Qubit荧光计的试剂盒
用于Qubit荧光计的Qubit试剂盒，配合简单的混合读取模式，均采用相同的操作方式。其中DNA和RNA分析试剂盒仅需孵育2分钟，而蛋白分析试剂盒需要15分钟 (图 3)。 Qubit 4荧光计自动保存您的数据，您可用USB数据线或U盘导出。 除了常规的 dsDNA，寡核苷酸，总RNA，小RNA和蛋白质的分析试剂盒，在 Ion Personal Genome Machine(PGM) 测序仪上进行测序之前，可使用专门的Ion Sphere Quality Control Kit在Qubit 4荧光计上进行Ion Sphere颗粒质量评估。

The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but may be used with any fluorometer or fluorescence plate reader. The Qubit® ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA but it will not detect contaminating protein or nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg/µL to 200 ng/µL providing an assay range of 1–200 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for ssDNA quantitation?
• For 1–20 samples: use this Qubit ssDNA Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT OliGreen ssDNA Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Sample Type (General): ssDNA

For Use With (Equipment): Qubit® Fluorometer

Quantitation Range: 1-200 ng

Number of Reactions: 100 reactions

Product Line: Quant-iT™

Product Size: 1 kit

Shipping Condition: Room Temperature

内容及储存

Store in refrigerator (2–8°C) and protect from light.

Note: 500 µL thin-walled PCR tubes are required but not included.