用于Qubit荧光计的Qubit试剂盒，配合简单的混合读取模式，均采用相同的操作方式。其中DNA和RNA分析试剂盒仅需孵育2分钟，而蛋白分析试剂盒需要15分钟 (图 3)。 Qubit 4荧光计自动保存您的数据，您可用USB数据线或U盘导出。 除了常规的 dsDNA，寡核苷酸，总RNA，小RNA和蛋白质的分析试剂盒，在 Ion Personal Genome Machine(PGM) 测序仪上进行测序之前，可使用专门的Ion Sphere Quality Control Kit在Qubit 4荧光计上进行Ion Sphere颗粒质量评估。

Product Line: Qubit™

Product Size: 100 assays

Invitrogen 1X dsDNA HS Assay Kit

Invitrogen 1X dsDNA HS Assay Kit

Green Features: Fewer resources used, Less waste

Shipping Condition: Room Temperature

内容及储存

Store in refrigerator at 2–8°C and protect from light.

The Qubit 1X dsDNA HS (High-Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The "1X" dsDNA assay kit provides reagent and buffer in a ready-to-use formulation. Simply add your sample (any volume between 1 µL and 20 µL is acceptable), add the Qubit 1X dsDNA HS reagent, and read the concentration using a Qubit 2.0, 3, 4, or Flex fluorometer. Common contaminants such as salts, free nucleotides, solvents, detergents, and protein are well-tolerated in the assay.

Notes:
1. The Qubit 1X dsDNA HS Assay works on Qubit 2.0, 3, 4, and Flex fluorometers. It will not work on the original Qubit 1.0 Fluorometer. Download the MyQubit .qbt file for the Qubit 2.0 or 3 fluorometer.
2. 500-mL thin-walled tubes displaying low auto-fluorescence (Cat. No. Q32856) are required but not included.