| 概述 |
|
| 别名 | Rabbit Anti-IL-1 Beta Polyclonal Antibody IL-1 β抗体;Catabolin; H1; IL-1β; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B_HUMAN; Interleukin-1 beta; IL-1 beta. |
| 宿主 | Rabbit |
| 特异性 | IL-1 Beta |
| 反应种属 | Mouse;Rat; |
| 预测反应种属 | Human;Dog;Horse;Rabbit; |
| 应用 | Rabbit Anti-IL-1 Beta Polyclonal Antibody WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） |
| 免疫原 | KLH conjugated synthetic peptide derived from human IL-1 Beta:161-269/269 |
| 性能 |
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| 浓度 | 1mg/ml |
| 纯化方法 | affinity purified by Protein A |
| 类型 | Polyclonal Antibody |
| 同种型 | IgG |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 存储溶液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 研究领域 | 细胞生物 免疫学 生长因子和激素 糖蛋白 |
| 靶标 |
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| 背景说明 | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]. |
| 细胞定位 | 分泌型蛋白
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| UniProt | P01584 |
| 实验结果图 |
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| Sample:
Brain (Mouse) Lysate at 40 ug
Intestine (Mouse) Lysate at 40 ug
Primary: Anti-IL-1 Beta at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 17/30 kD
Observed band size: 30 kD
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| Sample:
uterus (Mouse) Lysate at 40 ug
Primary: Anti-IL-1 Beta at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/30 kD
Observed band size: 27 kD
|
| Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (IL-1 Beta) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. |
| 温馨提示：本产品仅作科研实验使用，不支持临床等研究 |
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