PeproTech 内皮祖细胞培养基PeproGrow 700-EPC

PeproGrow™ EPC 内皮祖细胞培养基

英文名：PeproGrow™ EPC (Endothelial Progenitor Cell Media)

中文名：PeproGrow 内皮祖细胞培养基

PeproGrow EPC 适用培养内皮祖细胞。能良好维持内皮祖细胞的形态和功能，并提高内皮一氧化氮合酶活性。

产品订购信息

产品描述

PeproTech提供三种内皮细胞培养基，分别适用于体外培养：骨髓或外周血来源的内皮祖细胞（PeproGrow™ EPC ），大血管内皮细胞（PeproGrow™ MacroV）和微血管内皮细胞（PeproGrow™ MicroV）。这些培养基可以维持良好的内皮细胞形态和功能，并能提高内皮细胞关键的特异性标志物——内皮一氧化氮合酶（eNOS）的活性。由此，它们可为大血管、微血管内皮细胞及EPCs提供优良的细胞培养环境，且细胞生长率高于目前市售的其他培养基。

Product Details

Catalog Number:700-EPC

Description:

PeproTech offers three separate endothelial cell culture media formulations developed for the in vitro c*tion of: endothelial progenitor cells (EPC; PeproGrow EPC) derived from bone marrow or peripheral blood; endothelial cells from large vessels (PeproGrow MacroV); and endothelial cells from small vessels (PeproGrow MicroV). These media formulations maintain outstanding endothelial cell morphology and function, and increase the activity of endothelial nitric oxide synthase (eNOS), which account for a specific, crucial marker for endothelial cells. By doing this, the media provide an optimal cell culture environment for macrovascular and microvascular endothelial cells, as well as for EPC; growing cells at rates that exceed commercially available media. These media formulations are optimized for primary/progenitor human cells, but can also be used for bovine, porcine, rabbit, rat, and mouse EPC, or mature endothelial cells. 

PeproGrow EPC is supplied as a 500mL bottle of basal medium and a separate, frozen growth factor component.

PeproGrow EPC适用

PeproGrow EPC is recommended for Endothelial Progenitor Cells:

PeproGrow EPC推荐内皮祖细胞培养：
Human Endothelial Progenitor Cells (hEPC) 人内皮祖细胞
Mouse Endothelial Progenitor Cells (mEPC)  小鼠内皮祖细胞

PeproGrow EPC特征
Figures 1 and 2: EPC Proliferation. EPC were seeded onto fibronectin-coated plates, and incubated for 7 days in PeproGrow EPC basal medium + Growth Supplement-EPC (PeproTech) and a competitor’s medium. The proliferation ability of EPC was assessed 7 days after cell c*tion using the XTT assay according to the manufacturer protocol. The proliferation ability of EPC was expressed as the average of fluorescence intensity (O.D.) calculated using a plate reader for two independent assays run in triplicates. 

图一 EPC 扩增：左 其他品牌；右 Peprotech

内皮祖细胞扩增。内皮祖细胞接种在人纤连蛋白包被培养板上，分别使用PeproGrow EPC基础培养基+EPC生长因子（PeproTech）和其他品牌的培养基孵育7天。在细胞培养后7天用XTT分析培养基的内皮祖细胞扩增能力。

储存：基础培养基保存在2-8°C。生长因子使用前保存在 -20°C。

内皮一氧化氮合酶介绍

内皮一氧化氮合酶是一种一氧化氮合成酶。其他还有神经一氧化氮合酶和诱生性一氧化氮合酶。内皮一氧化氮合酶主要作用于血管内皮细胞的NO生成。NO对血管张力调节，细胞扩增，淋巴细胞粘连，血小板凝集都有重要作用。因此内皮一氧化氮合酶功能是健康心血管系统的基础。（英文：Wiki）

其他内皮细胞培养基

ScienCell 1001 内皮细胞培养基 现货供应（超链接）

PeproTech 内皮细胞培养基系列

PeproTech提供三种内皮细胞培养基试剂盒，分别适用于体外培养：骨髓或外周血来源的内皮祖细胞（PeproGrow™ EPC ），大血管内皮细胞（PeproGrow™ MacroV）和微血管内皮细胞（PeproGrow™ MicroV）。每款试剂盒由基础培养基和一管单独的内皮细胞生长添加剂组成。

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