TREM2 Effector Reporter Cell
| I. Background | |
TREM2是免疫球蛋白超家族的单次跨膜受体，主要表达于巨噬细胞、未成熟单核细胞 衍生的树突状细胞、破骨细胞和小胶质细胞上。TREM2 包含免疫球蛋白 V 型结构的胞外 域、跨膜域和没有信号传导基序的短胞内结构域。它通过 DNAX 激活蛋白 10 和 12 （DAP10 和 DAP12）发出信号。单个 TREM2 分子可以同时与 DAP10 和 DAP12 相互作 用。 | |
| II. Description | |
TREM2 Effector Reporter 报告基因药靶模型很好的模拟了体内 TREM2 的信号转导过程， 原理见下图所示。
Figure 1. TREM2 Effector Reporter 细胞模型原理图 | |
| III. Introduction | |
| Expressed gene: | TREM2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1 μg/ml Puromycin+10 μg/ml Blasticidin+800 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
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Figure 3. Dose Response of TREM2 Agonist Ab in TREM2 Effector Reporter Cells (C14). | |
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