hEpCAM/4T1
| I. Background | |||
| EpCAM（Epithelial Cell Adhesion Molecule，上皮细胞粘附分子，CD326）是一种跨膜 糖蛋白，在细胞的信号传导、粘附、细胞增殖分化、器官形成和维持中起着重要作用。 EpCAM 基因在人胚胎期除了胸腺组织以外的几乎所有的上皮组织中表达。在正常成人组 织中，EpCAM 在胃肠道、生殖系统和呼吸道的各种组织中的简单、假复层和移行上皮细 胞的基底外侧面表达。EpCAM 发生功能缺失性突变会导致先天性簇绒性肠病，在病理条 件下，EpCAM 在起源于上皮细胞的癌症中特异性表达。此外，EpCAM 在肺癌、乳腺癌、 宫颈癌和胰腺癌等多种癌症中高表达。 | |||
| II. Introduction | |||
| Host Cell: | 4T1 | ||
| Expressed gene: | hEpCAM | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | RPMI-1640+10%FBS+2 μg/ml puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mouse | ||
| Tissue: | Mammary gland | ||
| Morphology: | Epithelial | ||
| Growth Properties: | Adherent | ||
| IV. Representative Data | |||
Figure 1. Recombinant hEpCAM/4T1 stably expressing hEpCAM. | |||
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