CCL-155 RPMI 8226 人多发性骨髓瘤细胞 CCL-155 RPMI 8226 人多发性骨髓瘤细胞 ATCC® Number: CCL-155™ Designations: RPMI 8226 Isotype: lambda light chain Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: suspension Organism: Homo sapiens (human) Morphology: lymphoblast
CCL-155 Source: Organ: peripheral blood Disease: plasmacytoma; myeloma Cell Type: B lymphocyte; Cellular Products: immunoglobulin light chain Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. CCL-155 Isolation: Isolation date: June, 1966 Applications: transfection host Virus Susceptibility: Human poliovirus 1 Herpes simplex virus Vaccinia virus Vesicular stomatitis virus Antigen Expression: HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e + DNA Profile (STR): Amelogenin: X,Y CSF1PO: 12 D13S317: 11 D16S539: 9 D5S818: 11,13 D7S820: 9,10 THO1: 8 TPOX: 8,11 vWA: 16,18 Cytogenetic Analysis: Unstable karyotype in triploid range of 68-70 chromosomes. Two large marker chromosomes with terminal centromeres. Isoenzymes: G6PD, A Age: 61 years Gender: male Comments: There is no evidence of heavy chain production (cytoplasmic or secreted). Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.Do not allow the cell density to exceed 2 to 3 X 10(6) cells/ml. Interval: Maintain cell density between 5 X 10(5) and 2 X 10(6) viable cells/ml. Medium Renewal: Every 2 to 3 days Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 purified DNA:ATCC CCL-155D References: 1030: Matsuoka Y, et al. Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma. Proc. Soc. Exp. Biol. Med. 125: 1246-1250, 1967. PubMed: 6042436 26017: Moore GE, Kitamura H. Cell line derived from patient with myeloma. N.Y. State J. Med. 68: 2054-2060, 1968. PubMed: 5243066 43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375 CCL-155 |