| Designations: | SW 1353 [SW 1353, SW-1353] |
| Depositors: | A Leibovitz |
| Biosafety Level: | 1 |
| Shipped: | frozen |
| Medium & Serum: | See Propagation |
| Growth Properties: | adherent |
| Organism: | Homo sapiens （human） |
| Morphology: | fibroblast
|
| Source: | Organ: bone
Disease: chondrosarcoma |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| HTB-94 SW 1353 人骨肉瘤细胞 |
| Applications: | transfection host （Roche FuGENE® Transfection Reagents） |
| Antigen Expression: | Blood type O- |
| DNA Profile （STR）: | Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,12
D5S818: 10,11
D7S820: 9,11
THO1: 6,9
TPOX: 8,11
vWA: 16,17 |
| Cytogenetic Analysis: | hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident |
| Isoenzymes: | AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 2 |
| Age: | 72 years |
| Gender: | female |
| Ethnicity: | Caucasian |
| Comments: | The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian.
The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics.
A frozen ampule at passage 12 was received at the ATCC in January, 1982. |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week |
| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature |
| Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2008
recommended serum:ATCC 30-2020 |
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