P-K-BGLUBeta葡聚糖混联检测试剂盒

β-葡聚糖[混联]检测试剂盒

Colourimetric method for the determination of β-Glucan in
cereal grains, feed, foodstuffs, beverages and other materials

Principle:
                        (lichenase)
(1) β-Glucan + H2O → β-gluco-oligosaccharides

                                                (β-glucosidase)
(2) β-Gluco-oligosaccharides + H2O → D-glucose

                               (glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

                                                                               (peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
                                                                       quinoneimine + 4H2O
Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Total assay time:              ~ 100 min
Detection limit:                 0.5-100% of sample weight
Application examples:
Oats, barley, malt, wort, beer, food and other materials
Method recognition:    
AOAC (Method 995.16), AACC (Method 32-23.01), EBC (Methods
3.11.1, 4.16.1 and 8.11.1), ICC (Standard No. 166), RACI (Standard
Method) and CODEX (Type II Method)