20012Immunization grade chick type II collagen, 10 mg solution

Chondrex提供各种种类和类型的高度提纯ed软骨胶原蛋白、II型胶原蛋白,IX,XI,它适用于感应的胶原诱导的关节炎(CIA)和各种应用,如生物工程的artifi人工软骨和细胞培养。中情局可以诱导在啮齿类动物和非人灵长类与II型胶原免疫,但据报道,*也能够型诱导关节炎在小鼠和大鼠,因为α3(XI)连锁型胶原蛋白是与西安α1(II)链的二型胶原蛋白。

Collagen Induced Arthritis - Collagen

Chondrex provides various species and types of highly purifi ed cartilage collagen, type II, IX, and XI collagen, which are suitable for the induction of Collagen-Induced Arthritis （CIA） and various applications such as bioengineering of artifi cial cartilage and cell cultures. CIA can be induced in rodents and non-human primates by immunization with type II collagen, but it has been reported that type XI is also capable of inducing arthritis in mice and rats, since the alpha 3（XI） chain of type XI collagen is identical with the alpha 1（II） chain of type II collagen. Furthermore, it has been suggested that autoantibodies to type IX collagen may contribute to polychondritis development in mice. Choose the appropriate species and types of collagen for individual experimental purposes.

Type II Collagen

Species	Quantity	Catalog #
Bovine	10 mg, lyophilized	20021
Bovine	2 mg/ml x 5 ml	20022
Chick	10 mg, lyophilized	20011
Chick	2 mg/ml x 5 ml	20012
Goat	5 mg, lyophilized	20071
Human	1 mg, lyophilized	20051
Human	2 mg/ml x 0.5 ml	20052
Mouse	1 mg, lyophilized	20061
Mouse	2 mg/ml x 0.5 ml	20062
Porcine	10 mg, lyophilized	20031
Porcine	2 mg/ml x 5 ml	20032
Rat	5 mg, lyophilized	20041
Rat	2 mg/ml x 2.5 ml	20042
Sheep	5 mg, lyophilized	20081

Type IX Collagen

Species	Quantity	Catalog #
Bovine	5 mg, lyophilized	1072
Chick	5 mg, lyophilized	1071
Human	1 mg, lyophilized	1075
Porcine	5 mg, lyophilized	1073

Type XI Collagen

Species	Quantity	Catalog #
Bovine	5 mg, lyophilized	1082
Chick	5 mg, lyophilized	1081
Human	1 mg, lyophilized	1085
Porcine	5 mg, lyophilized	1083

Combination of MHC Types and Species of Collagen for Inducing CIA
Collagen-induced arthritis （CIA） can be induced in certain strains of mice, rats, and non-human primates by immunization with type II collagen emulsified with adjuvant. Susceptibility to CIA in rodents is linked to MHC class II molecules of animals, but also depends on the species of type II collagen used for immunization. Choose the appropriate species of type II collagen for inducing CIA according to the table below. Importantly, homologous type II collagen is not capable of inducing CIA by an ordinary immunization schedule.

 

Two Factors that should be considered by Users of Animal Models

Animal models have contributed to remarkable advances in the understanding of numerous human diseases. As invaluable as animal models are, some drawbacks do exist. One such drawback stems from the different sources of variability which can affect the outcome of the experiments. Therefore, a pilot study is generally recommended for first time users of all animal models. A brief discussion of the 2 main points of variability will be explained below.

1. Mouse Vendors

The genetic background of mice, even in the same strain, will vary from vendor to vendor and can affect how the animals respond to various reagents, thus impacting the results of your experiment （1）. Therefore, testing animals from different vendors using a defined protocol is recommended before proceeding with a large full-scale experiment.

2. Housing Conditions

The bacteria flora is very important to the health of the animal, including the proper functioning of the immune system （1）. Therefore, we recommend housing animals in Specific Pathogen Free （SPF） conditions rather than conventional conditions to avoid variability caused by bacteria.

Overall, special consideration of these main points of variability will aid the researcher in completing their experiments with less confounding results.

References

1. Ivanov II. et al. Induction of intestinal Th17 cells by segmented filamentous bacteria. Cell 139（3）: 485-498 （2009