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Size-Exclusion Chromatography (SEC) Columns for Separating Water-Soluble Polymers

Thermo Scientific^™ Acclaim^™ SEC-300 and SEC-1000 columns are a family of high-performance size-exclusion chromatography columns specifically designed for the separation of water-soluble polymers and oligomers. Acclaim SEC columns are available in two pore sizes, 300 Å and 1000 Å, and are optimized for separating a broad molecular weight range. Water-soluble polymers cover a wide range of highly varied families of products, both of natural or synthetic origin, and have numerous uses in pharmaceuticals, medications, cosmetics, food products, and industrial processes, to name a few.

Column features include:

Proprietary monodispersed, multipore, hydrophilic resin: no inflection points in calibration curve.
Two columns with different pore sizes offer wide linear range (100 to 1,000,000 Dalton).
Availability of small particle sizes packed in 4.6 × 300 mm dimension allows for high-resolution analysis with reduced solvent consumption.
Stable surface bonding with low column bleed and compatibility with UV, RI, MS, ELSD and Thermo Scientific Dionex Corona^™Charged Aerosol Detectors.

These columns are packed with spherical, monodispersed, hydrophilic polymethacrylate particles synthesized by multipore particle synthesis technology. Combined with the use of small particle sizes (5 µm for Acclaim SEC-300 and 7 µm for Acclaim SEC-1000), they provide high-resolution and good linearity of the calibration curve for accurate and reliable analysis of a variety of water-soluble polymers.

The Acclaim SEC-1000 uses polymethacrylate particles with a nominal pore size of 1000 Å, resulting in a broad linear calibration range from 1,000 to 1,000,000 Dalton. Therefore, it is suitable for characterizing high molecular weight polymers. The Acclaim SEC-300 uses polymethacrylate particles with a nominal pore size of 300 Å, giving a linear calibration range from 100 to 50,000 Dalton. As a result, this column offers good resolution for lower molecular weight polymers or oligomers.

Acclaim SEC Column Specifications

Acclaim SEC-300

Acclaim SEC-1000

Substrate

Hydrophilic polymethacrylate resin, Spherical

Particle size

5µm

7µm

Pore size

300 Å (multipore)

1000 Å (multipore)

Separation range for polyethylene oxides (PEO)

100–50,000 Daltons

1,000–1,000,000 Daltons

Exclusion limit for polyethylene oxides (PEO)

50,000–150,000 Daltons

3,000,000–7,500,000 Daltons

Operating pH range

2–12

Operating flow rate range

≤ 0.35 mL/min: 4.6 × 300 mm
≤ 1.0 mL/min: 7.8 mm i.d. formats

Column Technology

Acclaim SEC columns are packed with spherical, mono-dispersed, hydrophilic polymethacrylate particles synthesized by the multi-pore particle synthesis technology. Combined with the use of small particle sizes (5 µm for Acclaim SEC-300 and 7 µm for Acclaim SEC-1000), they provide high-resolution and good linearity of the calibration curve for accurate and reliable analysis for a variety of water soluble polymers.

Acclaim SEC columns offer two pore sizes, 300 Å and 1000 Å, to cover a broad molecular weight range. The Acclaim SEC-1000 uses polymethacrylate particles with a nominal pore size of 1000 Å, resulting in a wide linear calibration range from 1,000 to 1,000,000 Dalton (Figure 1). Thus it is suitable for characterizing high molecular weight polymers.

Analytical Columns
Acclaim SEC-1000, 7µm, Analytical (4.6 x 300 mm)	079724
Acclaim SEC-1000, 7µm, Analytical (7.8 x 300 mm)	079721
Acclaim SEC-1000, 7µm, Analytical (7.8 x 150 mm)	079722
Acclaim SEC-300, 5µm, Analytical (4.6 x 300 mm)	079723
Acclaim SEC-300, 5µm, Analytical (7.8 x 300 mm)	079725
Acclaim SEC-300, 5µm, Analytical (7.8 x 150 mm)	079726