EC-869Tris-Tricine-SDS PAGE Buffer （10X）

Tris-Tricine SDS-PAGE 电泳缓冲液（10X）

• Running Buffer for Small Proteins
• Variation on the Schagger/von Jagow System
• Substitute for Tris-Glycine-SDS in the Standard Laemmli Protocol

Catalog Number: EC-869

Tris-Tricine SDS-PAGE 电泳缓冲液（10X）

Discontinuous SDS-PAGE employing Tris-Glycine as the tank buffer resolves proteins down to about 15 kd. However, below this size, the proteins do not destack from the SDS micelles running through the gel with the buffer front. In order to resolve proteins in this size range, the Tris-Tricine system of Schagger and von Jagow （1987） was developed.

An Alternative to the Schagger and von Jagow System involves running Tris-Tricine Gels using National Diagnostics 10X Tris-Tricine-SDS Buffer （EC-869）. With National Diagnostics Tris-Tricine-SDS, you can extend the range of SDS-PAGE to resolve smaller proteins with minimal alteration of protocol.