| Organism: | TCC 9689 艰难梭菌Clostridium difficile 的详细介绍 Clostridium difficile （Hall and O'Toole） Prevot |
| Designations: | 90556-M6S [L.S. McClung 1780] |
| Depositor: | FE Clark |
| History: | F E Clark |
| Biosafety Level: | 2 |
| Shipped: | freeze-dried |
| Growth Conditions: | ATCC medium2107: Reinforced clostridial broth （modified）
Alternate medium 38: Beef liver medium for anaerobes
Temperature: 37.0°C
Atmosphere: Anaerobic |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| Cross References: | Nucleotide （GenBank） : AX110003 Sequence 736 from Patent WO0123604.
Nucleotide （GenBank） : AX110007 Sequence 740 from Patent WO0123604.
Nucleotide （GenBank） : AX110095 Sequence 828 from Patent WO0123604.
Nucleotide （GenBank） : AX111484 Sequence 2217 from Patent WO0123604.
Nucleotide （GenBank） : AX111505 Sequence 2238 from Patent WO0123604.
Nucleotide （GenBank） : AX111525 Sequence 2258 from Patent WO0123604.
Nucleotide （GenBank） : S51968 {16S/23S ribosomal DNA spacer region} [Clostridium difficile, ATCC 9689, Genomic, 271 nt]
Nucleotide （GenBank） : U36481 Clostridium difficile sporulation protein homolog （spo0A） gene, partial cds.
Nucleotide （GenBank） : U43514 Clostridium difficile putative spoIVB （spoIVB） gene, complete cds, and phosphorylation-activated transcription factor spo0A （spo0a） gene, complete cds.
Nucleotide （GenBank） : X94328 C.difficile spo0A gene. |
| Type Strain: | yes [36887] |
| Comments: | Presence of tcdA and tcdB genes confirmed by PCR. |
| Applications: | produces cytotoxin [832] [834] |
| Related Products: | genomic DNA: ATCC 9689D-5 |
| References: | 832: Rolfe RD, Finegold SM. Purification and characterization of Clostridium difficile toxin. Infect. Immun. 25: 191-201, 1979. PubMed: 478634
834: Florin I, Thelestam M. Intoxication of cultured human lung fibroblasts with Clostridium difficile toxin. Infect. Immun. 33: 67-74, 1981. PubMed: 6167521
36887: Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980.
16173174: Lemee L, et al. Multiplex PCR targeting tpi （triose phosphate isomerase）, tcdA （Toxin A）, and tcdB （Toxin B） genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42（12）: 5710-5714, 2004. PubMed: 15583303 |
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