CRL-1735 Lec1仓鼠卵巢细胞

ATCC® Number:  CRL-1735™       
Designations:  Lec1 [originally named Pro-5WgaRI3C]  
Depositors:   P Stanley  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: loosely adherent 
Organism: Cricetulus griseus （hamster, Chinese）  
Morphology: epithelial

 
Source: Organ: ovary 
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Applications: transfection host （Roche FuGENE® Transfection Reagents） 
Gender:  female  
Comments: This line is a mutant clone derived from the parental CHO clone Pro-5 （a proline auxotroph, see ATCC CRL-1781） by selection for resistance to wheat germ agglutinin. The cells lack GlcNAc glycosyl transferase so that N-linked carbohydrates are blocked at the Man5-GlcNAC2-Asn intermediate. The line may be useful for studying the effects of altered N-linked glycosylation on the function and compartmentalization of endogenous glycoproteins or glycoproteins introduced by viral infection or transfection of foreign DNA.
BR>The population contains Pro+ revertants at low frequency （approximay 1 in 100,000 cells）, and should be suitable for complementation studies which require the Pro- marker. 
The cells are proline auxotrophs and must be grown in a medium that contains proline （40 mg/L）. 
The population contains Pro+ revertants at low frequency （approximay 1 in 100000 cells）, and should be suitable for complementation studies which require the Pro- marker. 
Propagation:  ATCC complete growth medium: Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Temperature: 37.0°C 
Subculturing:  Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended 
Medium Renewal: 2 to 3 times per week 
Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Related Products: recommended serum:ATCC 30-2020 
References: 1201: Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798
23255: Stanley P, et al. Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine-- glycoprotein N-acetylglucosaminyltransferase activity. Proc. Natl. Acad. Sci. USA 72: 3323-3327, 1975. PubMed: 1059116 
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CRL-1735 Lec1仓鼠卵巢细胞

CRL-1735 Lec1仓鼠卵巢细胞

CRL-1735 Lec1仓鼠卵巢细胞