| Designations: | RKO-AS45-1 |
| Depositors: | MC Hollander, AJ Fornace |
| Biosafety Level: | 2 [Contains CMV viral sequences ] |
| Shipped: | frozen |
| Medium & Serum: | See Propagation |
| Growth Properties: | adherent |
| Organism: | Homo sapiens （human） |
| Morphology: | epithelial
|
| Source: | Organ: colon
Disease: carcinoma |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| CRL-2579 RKO-AS45-1 人结肠癌转基因细胞 |
| Oncogene: | p53 + （underexpressed） |
| Comments: | The RKO-AS45-1 cell line was generated from the colon carcinoma RKO （ATCC CRL-2577） cell line by transfection with the open reading frame portions of human cDNAs for GADD45 cloned into the expression vector pCMV. [51464]
The cells contain a stably integrated cytomegalovirus （CMV） promotor driven expression vector. The cells overexpress Gadd45 RNA and protein.
The GADD45 gene is p53-regulated and the Gadd45 protein interacts with proteins involved in both cell-cycle regulation and DNA repair.
The RKO-AS45-1 cell line has reduced ability to repair U.V.-induced DNA damage. [51464]
The RKO-AS45-1 cell line can be used together with its parental cell line, RKO （ATCC CRL-2577） to investigate the effects of Gadd45 on DNA repair, apoptosis and drug sensitivity. |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
| Subculturing: | Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Preservation: | culture medium 95%; DMSO, 5% |
| Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-2577 |
| References: | 51464: Smith ML, et al. Antisense GADD45 expression results in decreased DNA repair and sensitizes cells to u.v.-irradiation or cisplatin. Oncogene 13: 2255-2263, 1996. PubMed: 8950993
53491: Smith ML, et al. Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen. Science 266: 1376-1380, 1994. PubMed: 7973727
53492: Bae I, et al. An abnormality in the p53 pathway following gamma-irradiation in many wild-type p53 human melanoma lines. Cancer Res. 56: 840-847, 1996. PubMed: 8631022 |
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