CRL-1427MG-63 人骨肉瘤细胞

  MG-63 人骨肉瘤细胞

ATCC® Number:  CRL-1427™        
Designations:  MG-63  
Depositors:   A Billiau  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens （human）  
Morphology: fibroblast

MG-63 人骨肉瘤细胞 
Source: Organ: bone 
Disease: osteosarcoma 
Cellular Products: interferon  
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Applications: transfection host （Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents） 
Receptors: transforming growth factor beta （TGF-beta） RI, expressed 
transforming growth factor beta （TGF-beta） RII, expressed 
DNA Profile （STR）: Amelogenin: X,Y 
CSF1PO: 10,12 
D13S317: 11 
D16S539: 11 
D5S818: 11,12 
D7S820: 10 
THO1: 9.3 
TPOX: 8,11 
vWA: 16,19 
Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells. 
Age:  14 years  
Gender:  male  
Ethnicity:  Caucasian  
Comments: High levels of interferon production can be induced using polyinosinic - polycytidylic acid, cycloheximide and actinomycin D. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5% 
Temperature: 37.0°C 
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended 
Medium Renewal: 2 to 3 times per week 
Preservation:  Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003 
References: 1058: Billiau A, et al. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob. Agents Chemother. 12: 11-15, 1977. PubMed: 883813
23014: Takeuchi Y, et al. Relationship between actions of transforming growth factor （TGF）-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. PubMed: 7860639
32445: Yee A, et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996. PubMed: 8550604