TIB-161HuT 78 人T淋巴细胞白血病细胞

TIB-161 HuT 78 人T淋巴细胞白血病细胞

ATCC® Number:  TIB-161™        
Designations:  HuT 78  
Depositors:   AF Gazdar  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: suspension 
Organism: Homo sapiens （human）  
Morphology: lymphoblast

ATCC®编号：TIB-161™
名称：HuT 78
存款人：AF Gazdar
生物安全等级：1
发货：冷冻
中度和血清：见传播
生长特性：悬浮
生物：智人（人类）
形态学：淋巴母细胞

TIB-161 HuT 78 人T淋巴细胞白血病细胞 
Source: Disease: Sezary Syndrome 
Cell Type: cutaneous T lymphocyte; 
Cellular Products: interleukin 2 [1140] 
tumor necrosis factor alpha （TNF alpha） [23420]  
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
TIB-161  
Applications: transfection host （Nucleofection technology from Lonza） 
Receptors: interleukin 2 （IL-2）, expressed [1140] 
Tumorigenic: Yes  
Antigen Expression: CD4; Homo sapiens [22610] 
DNA Profile （STR）: Amelogenin: X,Y 
CSF1PO: 11,12 
D13S317: 8,12 
D16S539: 11,12 
D5S818: 11,12 
D7S820: 8,11 
THO1: 8,9 
TPOX: 8,9 
vWA: 14,15 
Age:  53 years adult  
Gender:  male  
Ethnicity:  Caucasian  
Comments: H9 （ATCC HTB-176） is a clonal derivative of HuT 78 [Pubmed: 2567177]. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5% 
Temperature: 37.0°C 
Subculturing:  Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp4 and 8 X 10 exp5 viable cells/ml. maintain cell density at less than 1 X 10 exp6 cells/ml. 
Medium Renewal: Two to three times weekly 
Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Doubling Time:  about 65 hours  
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2005
recommended serum:ATCC 30-2020
derivative:ATCC HTB-176 
References: 1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346
22484: Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177
22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013
23228: Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674
23420: O'Connell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285
32283: Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991
32396: Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128
32796: Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082 
TIB-161

TIB-161