CCL-2.1HeLa 229 人宫颈癌细胞

HeLa 229 人宫颈癌细胞

ATCC® Number:  CCL-2.1™      Designations:  HeLa 229  
Depositors:   JT Syverton  
Biosafety Level: 2 [Cells contain human papilloma virus （HPV-18） ]  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent （The line can be readily adapted to growth in suspension） 
Organism: Homo sapiens （human）  
Morphology: epithelial

HeLa 229 人宫颈癌细胞 
Source: Organ: cervix 
Disease: adenocarcinoma 
Cellular Products: keratin  
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Virus Susceptibility: Human adenovirus 7
Vesicular stomatitis virus
Human poliovirus 1
Human poliovirus 2
Human poliovirus 3 
Reverse Transcript: negative  
DNA Profile （STR）: Amelogenin: X 
CSF1PO: 9,10 
D13S317: 12,13.3 
D16S539: 9,10 
D5S818: 11,12 
D7S820: 8,12 
THO1: 7 
TPOX: 8,12 
vWA: 16,18 
Isoenzymes:  G6PD, A 
Age:  31 years  
Gender:  female  
Ethnicity:  Black  
HeLa Markers:  Y  
Comments: Relatively resistant to polioviruses. 
The cells are positive for keratin by immunoperoxidase staining. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5% 
Temperature: 37.0°C