CCL-244HCT-8 人回盲肠癌细胞

CCL-244 HCT-8 人回盲肠癌细胞

ATCC® Number:  CCL-244™       
Designations:  HCT-8 [HRT-18]  
Depositors:   A Sobrero  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens （human）  
Morphology: epithelial

CCL-244 HCT-8 人回盲肠癌细胞
Source: Organ: colon 
Disease: ileocecal colorectal adenocarcinoma 
Cellular Products: carcinoembryonic antigen （CEA） 0.5 ng/10 exp6 cells/10 days; alkaline phosphatase; keratin  
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CCL-244  
Tumorigenic: Yes  
Reverse Transcript: negative  
DNA Profile （STR）: Amelogenin: X,Y 
CSF1PO: 12 
D13S317: 8,11 
D16S539: 12,13 
D5S818: 13 
D7S820: 10,12,11.3 
THO1: 7,9.3 
TPOX: 8,11 
vWA: 18,19 
Isoenzymes:  AK-1, 1 
ES-D, 1-2 
G6PD, B 
GLO-I, 2 
Me-2, 1 
PGM1, 1 
PGM3, 1 
Age:  67 years adult  
Gender:  male  
Comments: The HCT-8 line is identical to the HRT-18 cell line. 
The cells are positive for keratin by immunoperoxidase staining. 
Propagation:  ATCC complete growth medium: RPMI 1640 medium with sodium pyruvate （1 mM）; 90%; horse serum, 10%
Temperature: 37.0°C 
Subculturing:  Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin, 0.02% EDTA in Hanks' BSS and incubate at 37C with the trypsin, EDTA for 10 to 15 minutes. Remove trypsin, EDTA, disperse cells in fresh medium, centrifuge, resuspend cells in medium and dispense into new flasks. 
Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended 
Medium Renewal: Every 2 to 3 days 
References: 1052: Tompkins WA, et al. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. Cancer Inst. 52: 1101-1110, 1974. PubMed: 4826581
26064: Nelson-Rees WA, et al. Distinctive banded marker chromosomes of human tumor cell lines. Int. J. Cancer 16: 74-82, 1975. PubMed: 1058173
32376: White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293

CCL-244