COLO-320 人结肠腺癌细胞 ATCC® Number: CCL-220™ Designations: COLO 320DM Depositors: GE Moore Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: mixed, adherent and suspension Organism: Homo sapiens (human) Morphology: rounded and refractile COLO-320 人结肠腺癌细胞 Source: Organ: colon Tumor Stage: Dukes' type C Disease: colorectal adenocarcinoma Cellular Products: serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Tumorigenic: Yes Reverse Transcript: negative DNA Profile (STR): Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11,12 D5S818: 12 D7S820: 9,12 THO1: 9 TPOX: 8,9 vWA: 15,18 Cytogenetic Analysis: modal number = 24; range = 49 to 61. The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined. Isoenzymes: ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 2 Age: 55 years Gender: female Ethnicity: Caucasian Comments: The line is negative for CEA, CSAp and colon antigen 3. The cells are weakly positive for keratins and vimentin. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Subculturing: Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol. Add 2.0 to 3.0 of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: Liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 References: 1045: Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117 22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 Related Links NCBI Entrez Search Make a Deposit Frequently Asked Questions Material Transfer Agreement Technical Support Related Cell Culture Products |