CCL-222 COLO 205 人结肠癌细胞 ATCC® Number: CCL-222™
Designations: COLO 205
Depositors: GE Moore
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Homo sapiens （human）
Morphology: epithelial CCL-222 COLO 205 人结肠癌细胞
Source: Organ: colon
Tumor Stage: Dukes' type D
Disease: colorectal adenocarcinoma
Derived from metastatic site: ascites
Cellular Products: carcinoembryonic antigen （CEA） 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 （IL-10, interleukin-10）
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CCL-222 COLO 205 人结肠癌细胞
Applications: transfection host （Roche FuGENE® Transfection Reagents）
Tumorigenic: Yes
Reverse Transcript: negative
DNA Profile （STR）: Amelogenin: X
CSF1PO: 11,12
D13S317: 10,12
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15
Cytogenetic Analysis: The stemline chromosome number is hypertriploid and 10-11 marker chromosomes （M1, M2, M4, M5, M6 and 5 others） were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.
Isoenzymes: ES-D, 1-2
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1-2
PGM3, 1-2
Age: 70 years
Gender: male
Ethnicity: Caucasian
Comments: This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. [1046]
The cells are CSAp negative （CSAp-）.
The line was derived from tissue from the same patient as COLO 201 （ATCC CCL-224）.
The cells are positive for keratin by immunoperoxidase staining.
COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Shake flask, transfer floating cells inot a new flask. Cells remaining attached may be removed using a standard trypsinization protocol. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same individual:ATCC CCL-224
References: 1046: Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251
22511: Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757
22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
41500: Bjork P, et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697
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