HTB-20 BT-474 人乳腺导管癌细胞

HTB-20 BT-474 人乳腺导管癌细胞

ATCC® Number:  HTB-20™        
Designations:  BT-474  
Depositors:   EY Lasfargues  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent, patchy （The cells form adherent patches of epithelial-like cells The patches are compact multilayered colonies that rarely become confluent） 
Organism: Homo sapiens （human）  
Morphology: epithelial

HTB-20 BT-474 人乳腺导管癌细胞

Source: Organ: mammary gland; breast 
Tissue: duct 
Disease: ductal carcinoma 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Virus Susceptibility: mouse mammary tumor virus （RIII-MuMTV） 
Tumorigenic: Yes  
DNA Profile （STR）: Amelogenin: X 
CSF1PO: 10,11 
D13S317: 11 
D16S539: 9,11 
D5S818: 11,13 
D7S820: 9,12 
THO1: 7 
TPOX: 8 
vWA: 15,16 
Cytogenetic Analysis: The cell line is aneuploid human female （XO usually）, with most chromosome counts in the hypertetraploid range. Several chromosomes （N11, N13, and N22） are absent, and others are clearly under-represented （N9, N14, and N15） with respect to the other normal chromosomes. Chromosome N7 tends towards over-representation in several karyotypes. Some of the missing normal chromosomes are represented by their involvement in the nine stable marker chromosomes: der（14）t（14;?）（q32,?）, unknown, iso（13q）, der（6）t（6;7）（q21;q21）, der（11）t（11;?）（14;?）, del（11）（p11）, unknown, unknown, der（2）t（2;?）（p21;?）. Several of the latter were reported by E. Lasfargues, et al. [22542] 
Isoenzymes:  AK-1, 1 
ES-D, 1 
G6PD, B 
GLO-I, 1 
Me-2, 0

PGM1, 1 
PGM3, 1 
Age:  60 years adult  
Gender:  female  
Ethnicity:  Caucasian  
Comments: The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Atmosphere: air, 95%; carbon dioxide （CO2）, 5% 
Subculturing:  Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended 
Medium Renewal: 2 to 3 times per week 
Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Related Products: recommended serum:ATCC 30-2020
Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 46-X
purified DNA:ATCC HTB-20D 
References: 22542: Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
22660: Lasfargues EY, et al. A human breast tumor cell line （BT-474） that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035
32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764