CCL-253NCI-H508人结肠直肠腺癌

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桑戈国际贸易（上海）有限公司
12元（具体成交价以合同协议为准）
2021-02-08 15:17:09
上海市
美国
384

【简单介绍】

品牌	其他品牌	供货周期	一周
应用领域	医疗卫生,环保,生物产业,石油

CCL-253 NCI-H508 人结肠直肠腺癌
ATCC® Number: CCL-253™
Designations: NCI-H508 [H508]
Depositors: AF Gazdar
Biosafety Level: 1
Shipped: frozen

【详细说明】

CCL-253 NCI-H508 人结肠直肠腺癌的详细介绍

ATCC^® Number:

CCL-253™

Designations:	NCI-H508 [H508]
Depositors:	AF Gazdar
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	floating aggregates of round cells with some attached cells
Organism:	Homo sapiens （human）
Morphology:	epithelial
Source:	Organ: cecum Disease: colorectal adenocarcinoma
Cellular Products:	carcinoembryonic antigen （CEA）, 1709 ng/ml per 10 exp6 cells per 10 days
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CCL-253 NCI-H508 人结肠直肠腺癌
Tumorigenic:	Yes
Antigen Expression:	Blood type A; Rh+; CA19-9 antigen
DNA Profile （STR）:	Amelogenin: X CSF1PO: 11,12 D13S317: 8,12 D16S539: 12 D5S818: 12 D7S820: 8,12 THO1: 9,9.3 TPOX: 8,11 vWA: 15,16
Cytogenetic Analysis:	modal number = 102; range = 71 to 131; most cells have DM's
Isoenzymes:	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1
Age:	55 years
Gender:	male
Ethnicity:	Caucasian
Comments:	This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:	*Subction Ratio:** A subction ratio of 1:2 to 1:4 is recommended Medium Renewal:* 3 times per week Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for two minutes. Remove the trypsin and let the flask sit at room temperature for 10 minutes （or until the cells detach）. Add fresh medium, aspirate and dispense into new flasks.
Preservation:	Culture medium, 95%; DMSO, 5%
References:	23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

CCL-253 NCI-H508 人结肠直肠腺癌


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