CRL-1620A172 人胶质母细胞瘤细胞

CRL-1620 A172 人胶质母细胞瘤细胞

ATCC® Number:  CRL-1620™

Designations:  A-172 [A172]  
Depositors:   DJ Giard  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens （human）  
Morphology: 
CRL-1620 A172 人胶质母细胞瘤细胞
Source: Organ: brain 
Disease: glioblastoma 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
CRL-1620  
Tumorigenic: No  
DNA Profile （STR）: Amelogenin: X,Y 
CSF1PO: 9,12 
D13S317: 11 
D16S539: 12 
D5S818: 11,12 
D7S820: 11 
THO1: 6,9.3 
TPOX: 8,11 
vWA: 20 
Age:  53 years  
Gender:  male  
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Subculturing:  Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 
Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended 
Medium Renewal: Every 2 to 3 days 
Preservation:  Freeze medium: culture medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2020 
References: 23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
23218: Giard DJ, et al. In vitro c*tion of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
32550: Debinski W, et al. Receptor for interleukin （IL） 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human  
CRL-1620 

CRL-1620