CRL-1932 786-O 人肾透明细胞腺癌细胞 ATCC® Number: CRL-1932™ Designations: 786-O [786-0] Depositors: RD Williams Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Homo sapiens (human) Morphology: epithelial CRL-1932 786-O 人肾透明细胞腺癌细胞 Source: Organ: kidney Disease: renal cell adenocarcinoma Cellular Products: parathyroid hormone (PTH) like peptide Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. CRL-1932 Applications: transfection host (technology from amaxa) Tumorigenic: Yes DNA Profile (STR): Amelogenin: X,Y CSF1PO: 10 D13S317: 8 D16S539: 12 D5S818: 9 D7S820: 11,12 THO1: 6,9.3 TPOX: 8,11 vWA: 15,17 Cytogenetic Analysis: hypertriploid; Y was present in 60% the cells examined Age: 58 years Gender: male Ethnicity: Caucasian Comments: This line was derived from a primary clear cell adenocarcinoma. The cells display both microvilli and desmosomes, and can be grown in soft agar. The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors. The peptide has an N terminal sequence similar to PTH, has PTH like activity, and has a molecular weight of 6000 daltons. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Subculturing: Protocol: CRL-1932 Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. CRL-1932 Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 References: 22648: Williams RD, et al. In vitro c*tion of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed: 1010528 22653: Williams RD, et al. In vitro c*tion of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102 CRL-1932 CRL-1932
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