QQ交谈
产品展示
Restriction EnzySigma-Aldrich Cloning Expres
- 公司名称：
- 更新时间：
- 所 在 地：
- 生产地址：
- 浏览次数：
- 上海辅泽商贸有限公司
- 2018-08-17 15:32:00
- 上海市
- 243
【简单介绍】
| 品牌 | Sigma-Aldrich | 货号 | Acc I (R6142) |
|---|---|---|---|
| 供货周期 | 一周 |
【详细说明】
Restriction Enzyme Buffer Reference
Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer for each enzyme (table 2).
Table 1
Activity (%) of each Sigma restriction enzyme in each Sigma buffer. The preferred buffer for each enzyme is highlighted in bold.
| Buffer | |||||
| Restriction Enzyme | SA (B7531) | SB (B8781) | SL (B3782) | SM (B3158) | SH (B3657) |
| Acc I (R6142) | 100 | 0-10 | 10-25 | 1-10 | 0-10 |
| Alu I (R6885) | 100 | 50-75 | 25-50 | 25-50 | 0-10 |
| Apa I (R4258) | 100 | 10-25 | 50-75 | 50-75 | 0-10 |
| Bam HI (R0260) | 100 | 100 | 75-100 | 100 | 25-50 |
| Bcl I (R8631) | 100 | 100 | 25-50 | 100 | 100 |
| Bgl II (R6377) | 100 | 100 | 25-50 | 100 | 100 |
| Bln I (R3131) | 25-50 | 50-75 | 0-10 | 25-50 | 100 |
| Bsm I (R3635) | 0-10 | 50-75 | 0-10 | 25-50 | 100 |
| Cfo I (R1761) | 75-100 | 50-75 | 100 | 50-75 | 25-50 |
| Cla I (R7763) | 100 | 100 | 75-100 | 100 | 100 |
| Dde I (R4256) | 50-75 | 75-100 | 25-50 | 25-50 | 100 |
| Dpn I (R8381) | 100 | 75-100 | 50-75 | 75-100 | 75-100 |
| Dra I (R4381) | 100 | 75-100 | 100 | 100 | 50-75 |
| Ecl XI (R3884) | 25-50 | 100 | 25-50 | 25-50 | 50-75 |
| Eco RI (R6265) | 100 | 100 | 25-50 | 50-75 | 100 |
| Eco RV (R2756) | 25-50 | 100 | 0-10 | 25-50 | 50-75 |
| Hae III (R5628) | 50-75 | 50-75 | 75-100 | 100 | 25-50 |
| Hind III (R1137) | 50-75 | 100 | 25-50 | 100 | 50-75 |
| Hpa I (R8507) | 100 | 25-50 | 25-50 | 50-75 | 25-50 |
| Kpn I (R1258) | 75-100 | 10-25 | 100 | 25-50 | 0-10 |
| Nco I (R8761) | 50-75 | 50-75 | 50-75 | 50-75 | 100 |
| Nde I (R5509) | 25-50 | 100 | 10-25 | 50-75 | 100 |
| Nhe I (R5634) | 100 | 25-50 | 100 | 100 | 10-25 |
| Not I (R8506) | 10-25 | 50-75 | 0-10 | 25-50 | 100 |
| Nsi I (R5884) | 50-75 | 100 | 10-25 | 50-75 | 100 |
| Pst I (R7023) | 25-50 | 25-50 | 10-25 | 25-50 | 100 |
| Pvu I (R1508) | 50-75 | 75-100 | 25-50 | 50-75 | 100 |
| Pvu II (R2631) | 25-50 | 25-50 | 25-50 | 100 | 25-50 |
| Rsa I (R4756) | 100 | 50-75 | 100 | 50-75 | 0-10 |
| Sac I (R5268) | 100 | 0-10 | 100 | 50-75 | 0-10 |
| Sal I (R0754) | 0-10 | 25-50 | 0-10 | 10-25 | 100 |
| Sau 3A (R0762) | 100 | 25-50 | 25-50 | 75-100 | 0-10 |
| Sca I (R5007) | 0-10 | 100 | 0-10 | 75-100 | 100 |
| Sfi I (R8256) | 25-50 | 25-50 | 75-100 | 100 | 25-50 |
| Sma I (R4503) | 100 | 0-10 | 0-10 | 0-10 | 0-10 |
| Spe I (R5257) | 75-100 | 75-100 | 75-100 | 100 | 100 |
| Sph I (R7135) | 50-75 | 75-100 | 25-50 | 100 | 75-100 |
| Ssp I (R6759) | 75-100 | 75-100 | 10-25 | 75-100 | 100 |
| Taq I (R9507) | 50-75 | 100 | 25-50 | 50-75 | 50-75 |
| Xba I (R7260) | 100 | 75-100 | 75-100 | 75-100 | 100 |
| Xho I (R6759) | 25-50 | 75-100 | 10-25 | 25-50 | 100 |
Table 2
The complete formulation of each Sigma Restriction Enzyme Buffer. The formulation of the storage buffer for each Sigma restriction enzyme is given on the catalog page (see links in table 1).
| Final concentration (mM) | |||||
| SA (B7531) | SB (B8781) | SL (B3782) | SM (B3158) | SH (B3657) | |
| Tris-acetate | 33 | ||||
| Tris-HCl | 10 | 10 | 10 | 50 | |
| Mg-acetate | 10 | ||||
| MgCl2 | 5 | 10 | 10 | 10 | |
| K-acetate | 66 | ||||
| NaCl | 100 | 50 | 100 | ||
| Dithioerythritol (DTE) | 1 | 1 | 1 | ||
| Dithiothreitol (DTT) | 0.5 | ||||
| 2-Mercaptoethanol (BME) | 1 | ||||
| pH at 37°C | 7.9 | 8.0 | 7.5 | 7.5 | 7.5 |
相关产品
- 73245Stains/Dyes
- SRP4754Signaling Molecule （Marker）
- T8694Receptors
- LYSISO1Organelle, Membrane-specific Kit
- M9195Dissociation
- SRP3190Growth Factors
- B0814Cytokines
- SRP3191Chemokines
- S1694Stemline®
- 51800CSpecialty Media
- R8758Classical Media
- SRP3196Vitamins, Hormones, Misc.
- SRP4595Signaling molecules
- A4941Proteins Peptides
- C3742Inhibitors
- BPQ24Bioactives/Supplements Kits
- C4428Bioactives/Supplements Enzymes
- A5228Antibodies against Proteins, Bioactives
- B9553Antibodies against Cytokines, Chemokines
- C2542Antibodies against Adhesion, Attachment
- M9195Inter / Intra Cellular Proteins, Enzymes
- H4777Extracellular Matrix Components
- S1694Stem Cell Culture Media
- DNAQFStem Cell Markers
- T8694Receptors for Stem Cell Biology
- NXTRACTOrganelle/Membrane-specific
- N7534Multiple Tissue Northern Blots
- A5691Antibodies against Proteins/Bioactives
- N4763General Stains/Dyes for Stem Cell
- SRP3296Signaling Molecule （Marker）
- DMN10RNA/DNA Isolation Products for Stem
- SHC001Stem Cell Transfection/Transduction
- C2542Antibodies against Adhesion/Attachment
- P8115核酸扩增
- SRP3181Bioactives/Supplements
- H0268Expression/Translation
- SRP3015Cytokines, Growth Factors, Chemokines
- I5270Antibodies against Cytokines/Growth
- P4236Inhibitors
- AMPD1Enzymes, Substrates, Inhibitors
产品搜索
请输入产品关键字：
联系方式
地址：上海市虹口区曲阳路910号复城国际1101
邮编：200437
联系人：郑经理
电话：13816982406,021-65010900
传真：021-65010900
手机：13816982406
留言：发送留言
网址：
商铺：https://www.chem17.com/st215208/
邮编：200437
联系人：郑经理
电话：13816982406,021-65010900
传真：021-65010900
手机：13816982406
留言：发送留言
网址：
商铺：https://www.chem17.com/st215208/