细胞活性检测试剂Sigma-Aldrich 细胞培养试剂
【简单介绍】
品牌 | Sigma-Aldrich | 货号 | 细胞活性检测试剂盒和试剂 |
---|---|---|---|
规格 | 生物缓冲液 | 供货周期 | 一周 |
主要用途 | 生物洗涤剂 |
经过细胞培养和昆虫细胞培养测试的试剂不同于研究级别的化合物,它们还经过额外的细胞培养测试。该测试旨在节省研究者在细胞培养应用之前筛选这些试剂所耗费的时间和精力。
抗生素
粘附和基质因子
生物缓冲液
生物洗涤剂
细胞解离
细胞冻存
细胞分离
细胞活性检测试剂盒和试剂
支原体检测试剂盒和试剂
其它试剂
【详细说明】
Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability
Assays to measure proliferation, viability and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper choice of an assay method depends on the number and type of cells used as well as the expected outcome. Assays for cell proliferation may monitor the number of cells over time, the number of cellular divisions, metabolic activity or DNA synthesis. Cell counting using viability dyes such as trypan blue or calcein-AM can provide both the rate of proliferation as well as the percentage of viable cells. 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a popular choice for measuring the number of cellular divisions a population has undergone. Upon entering the cell, CFSE is cleaved by intracellular esterases to form the fluorescent compound and the succinimidyl ester group covalently reacts with primary amines on intracellular proteins. Upon division, the fluorescence intensity of each daughter cell is halved which allows for the simple detection of the number of cell divisions by flow cytometry. Assays that measure metabolic activity are suitable for analyzing proliferation, viability, and cytotoxicity. The reduction of tetrazolium salts such as MTT and XTT to colored formazan compounds or the bioreduction of resazurin only occurs in metabolically active cells. Actively proliferating cells increase their metabolic activity while cells exposed to toxins will have decreased activity. | |||
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Assays & Reagents for Measuring Cytotoxicity, Proliferation & Viability Kits
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Cell Counting Equipment
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Reagents
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