上海辅泽商贸有限公司

微孔板迷你离心机,细胞离心涂片机,3111二氧化碳培养箱,赛洛捷克离心机,FC酶标仪

化工仪器网收藏该商铺

9

联系电话

13816982406

您所在位置：
上海辅泽商贸有限公司>>化学试剂>>生命科学-细胞培养>> DMEM / F12培养基Sigma-Aldrich DMEM / F12培养基
 QQ交谈
产品展示

DMEM / F12培养基Sigma-Aldrich DMEM / F12培养基

Sigma-Aldrich DMEM / F12培养基

点击浏览大图收藏此产品

  • 公司名称：
  • 更新时间：
  • 所 在 地：
  • 生产地址：
  • 浏览次数：
  • 上海辅泽商贸有限公司
  • 2018-07-12 15:56:50
  • 上海市
  • 2514

我要询价

【简单介绍】

品牌 Sigma-Aldrich 供货周期 一周
Classical Media and Salts
DME F12
During the past decade, researchers have reported the culture of a variety of cell lines in serum-free medium that contained, instead of serum,

【详细说明】

Classical Media and Salts

DME F12

  

During the past decade, researchers have reported the culture of a variety of cell lines in serum-free medium that contained, instead of serum, a supplement of nutrients, growth factors and hormones. For example, Mather and Sato reported the successful c*tion of Leydig cells and Sertoli cells in serum-free medium that contained insulin, transferrin,epidermal growth factor, leutinizing hormone or follicle stimulating hormone, somatomedin and growth hormone. Although the hormones and their concentrations are specific for the type of cell under study, the medium found to be most satisfactory for studies of this type was a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DME) and Ham's F-12 Nutrient Mixture. HEPES buffer is included in the formulation at a final concentration of 15 mM to compensate for the loss of buffering capacity incurred by eliminating serum.

Product No.ProductDescriptionApplication
D6421Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With 15 mM HEPES and sodium bicarbonate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Without L-glutamine, supplement with 0.365 gm/L L-glutamine
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Liquid form of original 1:1 mixture of DMEM/Ham's F-12, with one important exception.  Pyridoxal, which is an aldehyde, has been replaced with pyridoxine.  This modification has been shown to improve the stability of the medium.

Suitable for growth of wide range of cell types in  low serum conditions.
D8437Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With 15 mM HEPES, L-glutamine and sodium bicarbonate
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Same as D6421, except the L-glutamine is added to the medium at time of manufacture.
D8900Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Powder
  • With L-glutamine and 15 mM HEPES
  • w/o sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 15.6 grams of powder per liter of medium
  • Cell culture tested
Powder form of original 1:1 mixture of DME/Ham's F-12. Suitable for growth of a wide range of cell types in low serum conditions.
D6905Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham Hybi-Max.
  • Powder
  • With L-glutamine and 15 mM HEPES
  • w/o sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 15.6 grams of powder per liter of medium
  • Cell culture tested
Formulation is identical to D8900. This formulation has been tested with and is recommended for use with hybridoma cells.
D6434Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With 15 mM HEPES and sodium bicarbonate
  • Without phenol red and L-glutamine, supplement with 0.365 gm/L L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Sterile-filtered
Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.

Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.
D2906Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Powder
  • With L-glutamine and 15 mM HEPES
  • Without phenol red and sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 15.6 grams of powder per liter of medium
  • Cell culture tested
Suitable for growth of a wide range of cell types in low serum conditions.

The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.
D6559Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With sodium bicarbonate
  • Without phenol red and L-glutamine, supplement with 0.365 gm/L L-glutamine
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.

Suitable for growth of a wide range of cell types in low serum conditions. The absence of phenol red makes this a preferred medium for clonal and stem cell culture.

The absence of HEPES allows the investigator to optimize the buffering system.
D8062Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Liquid
  • With L-glutamine and sodium bicarbonate
  • Without HEPES
  • With pyridoxine (substitutes pyridoxine HCl for pyridoxal HCl)
  • Endotoxin tested
  • Cell culture tested
  • Sterile-filtered
Same as D8437, except that HEPES has not been added to the formulation.

Allows the investigator to optimize the buffering system.
D0547Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham
  • Powder
  • With L-glutamine and trace elements
  • Without HEPES and sodium bicarbonate, to be supplemented with 1.2 gm/L of sodium bicarbonate
  • Formulated at 12.1 grams of powder per liter of medium
  • Cell culture tested
Powder form of original 1:1 mixture of DME/Ham's F-12, supplemented with trace elements.

Trace element supplementation of the original formula (D8900) is required for the compley serum free culture of a wide range of cell types.
D9785Dulbecco's Modified Eagle's Medium/Nutrient F-12 Ham Base
  • Powder
  • With 15 mM HEPES
  • Without L-glutamine, L-leucine, L-lysine, L-methionine, calcium chloride, magnesium chloride, magnesium sulfate, phenol red and sodium bicarbonate
  • Supplement with 0.1545 gm/L calcium chloride; 0.365 gm/L L-glutamine; 0.05905 gm/L L-leucine, 0.09125 gm/L L-lysine; 0.0612  gm/L magnesium chloride, 0.04884 gm/L magnesium sulfate, 0.0172 gm/L L-methionine, and 1.2  gm/L sodium bicarbonate
  • Formulated at 14.8 grams of powder per liter of medium
  • Cell culture tested
The most basic formulation offered.  This formulation is used by investigators who want to start with the essential components of (1:1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with label-tagged amino acids.

 

    
留言框
感兴趣的产品： *
留言内容：
您的姓名： *
您的单位：
联系电话： *
微信：
常用邮箱：
详细地址：
省份： *
验证码： * =   请输入计算结果（填写阿拉伯数字），如：三加四=7
      

相关产品

产品搜索

请输入产品关键字：

产品分类品牌
联系方式
地址：上海市虹口区曲阳路910号复城国际1101
邮编：200437
联系人：郑经理
电话：13816982406,021-65010900
传真：021-65010900
手机：13816982406
留言：发送留言
网址：
商铺：https://www.chem17.com/st215208/
| 商铺首页 | 公司档案 | 产品展示 | 供应信息 | 公司动态 | 询价留言 | 联系我们 | 会员管理 |
化工仪器网 设计制作，未经允许翻录必究.Copyright(C) https://www.chem17.com, All rights reserved.
以上信息由企业自行提供，信息内容的真实性、准确性和合法性由相关企业负责，化工仪器网对此不承担任何保证责任。
温馨提示：为规避购买风险，建议您在购买产品前务必确认供应商资质及产品质量。
二维码 在线交流

扫一扫访问手机站