紫外灯用于微针制备的固化

近日，天津医科大学与天津大学在《Nature Communications 》( IF 14.7 )期刊发表文献《Microorganism microneedle micro-engine depth drug delivery》，文献中的实验使用了路阳仪器上海公司生产的LUYOR-3109台式高强度紫外线灯，实验采用了LUYOR-3109 365nm台式大功率紫外线灯用于微针制作过程中的固化。

LUYOR-3109 365nm紫外光源安装了27w 大功率365nm led，通过二次光学透镜进行聚焦，紫外光强度超过了传统400w高压汞灯的辐照强度，上市以来，LUYOR-3109已经广泛用于科研院所的科学实验室的紫外辐照，目前，使用LUYOR-3109发表的文献近百篇。如果您实验需要一台大功率、高强度的365nm紫外光源，路阳仪器上海公司提供样机免费试用。

文献摘要：

作为一种透皮给药方法，微针具有微创、无痛和精确的原位治疗。然而，目前的微针依赖于被动扩散，导致药物渗透不可控。为了克服这个问题，我们开发了一种气动微针贴剂，它使用活的产气肠杆菌作为微引擎来主动控制药物输送。这些微生物产生气体，将药物驱使进入更深的组织，可调节的葡萄糖浓度可以精确控制该过程。我们的结果表明，这种微生物驱动的系统将药物输送深度增加了 200% 以上，达到皮肤以下 1000 μm。在银屑病动物模型中，该技术有效地将骨化三醇输送到皮下组织，从而快速缓解症状。这项创新解决了传统微针的 局限性，提高了药物效率、透皮渗透性，并引入了按需受控药物输送的创造性范式。

Preparation method of single-layer bacteria-carrying microneedles

First, a solution was prepared by mixing PEGDA and anhydrous ethanol in a 7:3 ratio to make a 10 mL solution. Then, 100 µL of HMPP (1%, v/v) was added to the solution, and it was thoroughly mixed to obtain the substrate solution. Next, a solution of Enterobacter Aerogenes ATCC 13048(E.A.) was placed in a centrifuge tube, and the centrifuge was set to 3000 rpm for 15 min. The resulting centrifuged pellet was thoroughly mixed with the substrate solution to obtain a bacteria-loaded substrate material. Different proportions of glucose solution can be added to the substrate solution as needed to obtain microneedle patches with varying substrate concentrations. Subsequently, a microneedle fabrication mold (microneedle width 200 µm, needle length 500 µm) was taken, and 300 µL of the bacteria-loaded substrate material was added to it. The mold was placed in a vacuum pump for 20 seconds to remove any bubbles from the microneedle mold’s needle array. The mold was then removed, and if necessary, it was exposed to a UV lamp(LUYOR-3109, 27w LED lamp) for 5 s to cure, resulting in complete microneedles. If there was any remaining liquid not drawn into the mold’s pores, an additional 300 µL of the substrate solution was added to fill the mold. A UV lamp was used to cure it for 5 s, and the microneedles were demolded to prepare complete bacteria-loaded microneedles.

文中使用的设备，另外配置多种不同款式紫外灯，中高强度，强度自由调节，物美价廉