Azido-PEG4-NHS Ester叠氮基-PEG4-NHS 酯是一种标记试剂，可用于通过稳定的酰胺键修饰具有叠氮基团的伯胺基团（例如赖氨酸残基的侧链或氨基硅烷包被的表面）。叠氮化物 （N3） 基团通过 CuAAC 与末端炔烃反应反应，通过无铜点击反应与应变环辛炔（例如 DBCO 或 BCN 化合物）反应，或通过称为 Staudinger 化学的机制与磷化氢标记分子反应，从而实现生物样品中衍生化分子的高效和特异性偶联。

Azido-PEG4-NHS Ester is a labeling reagent that can be used to modify primary amine groups (e.g., a side chain of lysine residues or aminosilane-coated surfaces) with an azido group via a stable amide bond. The azide (N3) group reacts with terminal alkynes via CuAAC, strained cyclooctynes (e.g. DBCO or BCN compounds) via Cu-free click reaction or with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.