单层应力加载单个细胞应力刺激系统

细胞被均匀地限制/压缩在两个亚微米分辨率的两个平行表面之间。

不同的限制高度（例如1um – 300um）

允许长期细胞培养和细胞增殖，同时保持对封闭的控制

与高分辨率光学显微镜系统兼容

可以处理足够多的细胞以进行完整的基因表达分析

可与生物功能化的微结构化底物和/或不同的基质（几何形状控制）结合使用

可以与凝胶结合（硬度控制）

兼容任何细胞培养底物（培养皿至96孔板）

产品：

应用：

Cell migration 2.5D, migration and interaction of non-adhesive cells, cell squeezing, imaging of flat cells (organelles aligned in 2D), super-resolution video-microscopy (organelles move less), contractility assay, etc

Confinement illustration
HeLa cells: not confined, 5 ?m, 3 ?m.

Explore examples of applications


> Cancer invasiveness assay: Quantification of migration behaviors and migration transitions

> Cancer aggressiveness assay: Quantification of contractility of somatic or cancer cells

> Endocytosis assay: Improved observation of events taking place at the membrane

> Exocytosis assay: Improved observation of events taking place at the apical membrane

> Frustrated phagocytosis: Characterization of the mechanism

> Immune system in a well: 2D migration and interaction of non-adherent immune cells

> Immune cells interaction: 2D interaction of non-adherent immune cells

> Mitotic assembly assay: Quantification of mitotic spindle disorders

> Quantitative cell migration assay: Fast and fine analysis of cell migration properties
 

文献：

PUBLICATIONS

• Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
  Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672
• Actin flows induce a universal coupling between cell speed and cell persistence
  P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386
• Geometric friction directs cell migration
  M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101
• Mitotic rounding alters cell geometry to ensure efficient spindle assembly
  O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283
• Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
  M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414
• Exploring the Function of Cell Shape and Size during Mitosis
  C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169
• Methods for Two-Dimensional Cell Confinement
  M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29