来自食用海藻的阿塞诺脂分析

来自食用海藻的阿塞诺脂

Arsenolipids from Edible Seaweed by LC-ICP-MS and LC-ESI-MS

Conditions
Column: ACE 3 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-111-1546
Mobile Phase:

A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeOH

Flow Rate: 1 mL/min
Injection: 100 μL
Temperature: 45 °C
Detection: Split ratio: 75% ESI-MS: 25% ICP-MS
Thermo Scientific Element 2 ICP-MS
Mode: Organic mode
Medium resolution
Thermo Scientific Orbitrap Discovery
Positive ESI mode
Spray voltage: 4.5 kV
Capillary temperature: 320 °C
Capillary voltage: 42 V

Arsenic-containing hydrocarbon:

m/z 389 [M + H]+ for C21H46AsO

Arsenic-containing phospholipids:
 m/z 959 [M + H]+ for C45H89AsO14P (C16:0/C16:0)
m/z 987 [M + H]+ for C47H93AsO14P (C18:0/C16:0)
m/z 1015 [M + H]+ for C49H97AsO14P (C20:0/C16:0)