犬溶菌酶（LYS）ELISA试剂盒实验原理

Canine lysozyme(LYS)ELISA Kit
犬溶菌酶(LYS)ELISA试剂盒实验原理：
本试剂盒仅供研究使用，用于测定犬血清，血浆及相关液体样本中溶菌酶（LYS）含量。应用双抗体夹心法测定标本中犬溶菌酶（LYS）水平。用纯化的犬溶菌酶（LYS）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入溶菌酶（LYS），再与HRP标记的LYS抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的溶菌酶（LYS）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中犬溶菌酶（LYS）浓度。
Principle of the assay:
This kit allows for the determination of LYS concentrations in canine serum, blood plasma, and other biological fluids.Assay canine LYS level in the sample，use Purified canine LYS antibody to coat microtiter plate wells, make solid-phase antibody, then add LYS to wells, Combined LYS antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LYS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
试剂盒供应商：上海华壹生物科技有限公司