Cytotoxicity of aluminum oxide nanoparticles on Allium cepa root tip—effects of oxidative stress gen-资料下载-美国布鲁克海文仪器公司

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The commercial usage of Al2O3 nanoparticles

(Al2O3 NPs) has gone up significantly in the recent times,

enhancing the risk of environmental contamination with these

agents and their consequent adverse effects on living systems.

The current study has been designed to evaluate the cytogenetic

potential of Al2O3 NPs in Allium cepa (root tip cells) at a

range of exposure concentrations (0.01, 0.1, 1, 10, and

100 μg/mL), their uptake/internalization profile, and the oxidative

stress generated. We noted a dose-dependent decrease

in the mitotic index (42 to 28 %) and an increase in the number

of chromosomal aberrations. Various chromosomal aberrations,

e.g. sticky, multipolar and laggard chromosomes, chromosomal

breaks, and the formation of binucleate cells, were

observed by optical, fluorescence, and confocal laser scanning

microscopy. FT-IR analysis demonstrated the surface chemical

interaction between the nanoparticles and root tip cells.

The biouptake of Al2O3 in particulate form led to reactive

oxygen species generation, which in turn probably contributed

to the induction of chromosomal aberrations.