商品信息

产品英文名称	Human Transferrin protein
产品中文名称	重组人转铁蛋白（TF）
别名	PRO2086 Protein; TFQTL1 Protein; HEL-S-71p Protein, Human; PRO1557 Protein; Transferrin Protein,Siderophilin, partially saturated

商品属性

标签	无标签
种属	人
序列	Amino acid sequence derived from Human Transferrin protein isoform (P02787, Met 1-Pro 698) with a polyhistidine tag at the C-terminus was expressed.
活性	检测进行中
蛋白长度	重组人转铁蛋白由690个氨基酸组成，预测分子量为76.6 kDa。 在还原条件下，SDS-PAGE中人转铁蛋白的表观分子量约为70-100 kDa。
纯度	≥ 95 % ，使用SDS-PAGE检测
特点&优势	采用 LAL 法检测，每 1 μg 蛋白质中的 EU 含量小于 1.0。
产品形式	冻干粉，冻干缓冲液为无菌的 PBS，pH 7.4。
基因ID	7018
蛋白序列链接	/uniprot/P02787
使用中注意事项	开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。
保存建议	本产品为冻干粉形式，建议：将冻干粉的重组人转铁蛋白（TF）产品保存于-20℃以下的干燥环境中。本产品溶解之后，可在4℃保存2-7天，长期使用请置于-20℃以下。为长期保存，推荐添加载体蛋白（0.1% HSA或者BSA），并酌情分装保存，避免反复冻融。
运输条件	常温运输
警告	本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何或许可证。对于使用本产品可能发生的侵权或其他违规行为，我们不承担任何责任。

附加信息

背景

Transferrin (Tf),is an iron transporter, which is mainly biosynthesized in the liver, and it can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc.The glycans of serum-type Tf in the CSF are similar to those of Tf in serum. Biochemical analyses reveal that the apparent molecular size of brain-type Tf is smaller than that of serum-type Tf, and that hydrophobic patches are exposed on brain-type Tf as demonstrated by hydrophobic probe binding studies. Research indicates that brain-type Tf levels is decreased in idiopathic normal pressure hydrocephalus, in which CSF production is suspected to decrease, while brain-type Tf increased in spontaneous intracranial hypotension, in which CSF production is suspected to increase. These results suggest that brain-type Tf could be a biomarker of altered CSF production.

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