Gibco Advanced RPMI 1640 培养基包含的成分可减少50-90%的 FBS 添加剂，这既能降低成本，又能减少批次间血清的变化，提高细胞培养的一致性。

Gibco RPMI 1640 培养基被发现适用于多种哺乳动物细胞，包括 HeLa、Jurkat、MCF-7、PC12、PBMC、星形胶质细胞和癌细胞。

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.ⁱ Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.

The complete formulation is available.

Gibco™ Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

ⁱAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.