含有 GlutaMAX 添加剂的 Gibco RPMI 1640 培养基含有稳定形式的 L-，在细胞培养时不会自发降解，可以防止氨沉积，改善细胞健康状况并提高细胞活性。

RPMI 1640 培养基含有还原剂、、12 以及 PABA，这些维生素是 Eagle 必需培养基和 Dulbecco 改良 Eagle 培养基所不具备的。此外，RPMI 1640 培养基含有高浓度维生素肌醇和。由于 RPMI 1640 培养基不包含蛋白质、脂质或生长因子，因此通常需要补充胎牛血清（FBS）。RPMI 1640 培养基使用的缓冲系统需要在5–10%的 CO₂ 环境条件下来维持生理 pH 值。

赛默飞世尔科技针对多种细胞培养应用，对 RPMI 培养基组分进行了大量的改良。

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.