CREBBP TR-FRET Assay Kit 返回列表页

The CREBBP TR-FRET Assay Kit requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward: a terbium-labeled donor, dye-labeled acceptor, CREBBP protein, an acetylated BET Bromodomain Ligand, and test inhibitor are incubated together for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Illustration of the assay principle: The Donor Terbium-labeled donor binds to GST-tagged CREBBP. The acetylated (Ac) BET Bromodomain Ligａnd is labeled with biotin, which allows the dye-labeled streptavidin acceptor to bind to the ligand. The TR-FRET signal is generated by proximity induced upon interaction of CREBBP with the acetylated ligand. Inhibitors of the interaction will prevent TR-FRET from happening, decreasing the signal in a dose-dependent manner.