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The BACE1 Assay Kit is designed to measure BACE1 activity for screening and profiling applications using a labeled peptide substrate in a homogeneous fluorogenic assay.
The peptide substrate contains a fluorescence Donor that is quenched by an Acceptor when the peptide is intact. Upon BACE1-mediated cleavage the two peptide fragments pull apart, releasing the highly fluorescent peptide fragment from quenching. Therefore, the fluorescence intensity increases proportionally to the activity of BACE1.
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