MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

Fluo-3AM

CAS No. : 121714-22-5

产品活性：Fluo-3 AM is a fluorecent Ca²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: 1. Preparation of Fluo-3 AM working solution
1.1 Preparation of the stock solution
Dissolve 1 mg Fluo-3 AM in 135 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃?or -80℃?away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Fluo-3 AM working solution
Dilute the stock solution in HBSS to obtain 1-10 μM of? working solution.
Note: Please adjust the concentration of Fluo-3 AM?working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4℃?for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×10⁶/mL
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃?for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.
Storage
-20℃, 1 year
Protect from light

相关产品：MeOSuc-Ala-Ala-Pro-Val-pNA  |  BDP 558/568 amine  |  AC-green  |  Naphthalene-1,2-dicarbaldehyde  |  Rhodamine 800  |  Vari Fluor 532 SE  |  Acridine Orange hydrochloride  |  N-(7-Nitrobenzofurazan-4-yl)phallacidin  |  Biotin-11-UTP  |  BODIPY FL SSE  |  1,2-Diamino-3,4-ethylenedioxybenzene  |  Sulfo-Cyanine5.5 NHS ester tripotassium  |  BODIPY 505/515  |  3-Acetylumbelliferyl β-D-Glucopyranoside  |  Ac-DNLD-AMC  |  Mucicarmine  |  7-Aminocoumarin  |  NEP  |  Sulfo-cyanine3 azide sodium  |  Dihydrorhodamine 123  |  N-Acryloyl-1-pyrenebutylamine  |  TSPP tetrasodium  |  Dabcyl acid  |  7-Diethylamino-4-methylcoumarin  |  9-(2-Carboxy-2-cyanovinyl)julolidine  |  RT-NH2  |  Coumberol  |  Carmoisine  |  4-Methylumbelliferyl-N-acetyl-α-D-glucosaminide  |  MitoMark Red I

热门产品线：重组蛋白  |  化合物库  |  天然产物  |  荧光染料  |  PROTAC  |  同位素标记物  |  寡核苷酸  |  抗体  |  点击化学

Trending products：Recombinant Proteins  |  Bioactive Screening Libraries  |  Natural Products  |  Fluorescent Dye  |  PROTAC  |  Isotope-Labeled Compounds  |  Oligonucleotides  |  Antibodies  |  Click Chemistry

品牌介绍:
•   MCE (MedChemExpress) 拥有200 多种*仅有化合物库，我们致力于为*科研客户提供前沿的高品质小分子活性化合物；
•   50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领；
•   产品种类涵盖各种重组蛋白，多肽，常用试剂盒 ，更有 PROTAC、ADC 等特色产品，广泛应用于新药研发、生命科学等科研项目；
•   提供虚拟筛选，离子通道筛选，代谢组学分析检测分析，药物筛选等专业技术服务；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种顶级期刊及制药 Patent 收录了MCE客户的科研成果；
•   专业团队跟踪新的制药及生命科学研究进展，为您提供*新的活性化合物；
•   与世界各大制药公司及*科研机构建立了长期的合作。