当前位置:上海富雨生物科技有限公司>>细胞库 / 细胞培养>>普通细胞>> 15P-1小鼠睾丸上皮细胞(附
小鼠睾丸上皮细胞15P-1
种属 | 小鼠 |
别称 | 15P-1 |
组织来源 | 睾丸 |
疾病 | 转化细胞系 |
传代比例/细胞消化 | 1:2传代,消化2-3分钟 |
培养基配置 | DMEM养基;10%胎牛血清;1%双抗 |
简介 | 15P-1细胞来源于在生精上皮中表达多瘤病毒(PyLT)大T蛋白的转基因小鼠的睾丸细胞。该细胞表现出支持细胞 (Sertoli cells)的特征,包括转录威尔姆斯肿瘤(WT1)和Steel基因。据报道,15P-1培养物呈现出吞噬活性,乳 胶颗粒的内吞和死细胞的清除就证明了这个吞噬活性。与生殖细胞的相互作用增强了15P-1细胞的吞噬活性。与二倍 体的减数分裂前期的生殖细胞共培养时,该细胞支持精原细胞经减数分裂和减数分裂后分化成为表达(Prm- 1)基因的单倍体精子。 |
形态 | 上皮细胞样 |
生长特征 | 贴壁生长 |
倍增时间 | 每 周 2 至 3 次 |
保藏机构 | ATCC;CRL-2618 |
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