当前位置:上海富雨生物科技有限公司>>细胞库 / 细胞培养>>普通细胞>> NR8383大鼠肺泡巨噬细胞(
大鼠肺泡巨噬细胞NR8383
属 | 大鼠 |
别称 | NR-8383; NR 8383; NR8383.1; AgC11x3A; Normal Rat, August 3, 1983 |
组织来源 | 肺;肺泡 |
疾病 | 自发永生细胞系 |
传代比例/细胞消化 | 1:2传代,悬浮部分离心收集(1000RPM,5分钟) ,贴壁部分消化1-2分钟 |
培养基配置 | Ham's F-12K培养基; 20%胎牛血清;1%双抗 |
简介 | NR8383细胞来源于肺灌洗时的正常大鼠肺泡巨噬细胞 ,NR8383细胞在Gerbil肺细胞连续培养液存在下培养了8-9个 月。随后 ,NR8383细胞不再需要外源生长因子。通过有限稀释法 ,从单个细胞克隆并亚克隆NR8383细胞 ,并3次用 软琼脂亚克隆。NR8383细胞表现出巨噬细胞的特性:吞噬酵母多糖和铜绿、非特异性脂酶活性、Fc受体、氧化降 解;分泌IL-1、TNF-β和IL-6 ,可重复地响应外源生长因子。NR8383细胞响应 ,分泌TNF-β前体。 NR8383细胞在刺激下 ,TNF-β mRNA表达也上升。NR8383细胞对内毒素敏感 ,1-10ng/ml的LPS水平抑 制增生达50%。即使达到0.001mg/ml的水平 ,LPS抑制还是无毒且在后续过程中可逆的。NR8383细胞提供了高响 应的肺泡巨噬细胞的均一来源 ,可以用于体外研究巨响细胞相关活性 |
形态 | 巨噬细胞样 |
生长特征 | 贴壁 ,悬浮混合生长 |
倍增时间 | 每周 2 至 3 次 |
培养条件 | 气相:空气 ,95% ;二氧化碳 ,5%。 温度:37摄氏度 ,培养箱湿度为70%-80%。 |
冻存条件 | 冻存液:90%FBS ,DMSO 10%, 或使用非程序冻存液:货号JY-H040 |
保藏机构 | ATCC; CRL-2192 |
备注 | 该细胞为半悬浮和半贴壁细胞 ,悬浮细胞离心收集 ,贴壁细胞消化处理 |
产品使用 | 于科学研究 ,不可作为动物或人类疾病的治疗产品使用。 |
cowpea-wheat crop sequence. Appl. Soil Ecol. 157, 103764
Galvez, A., Sinicco, T., Cayuela, g.L., gingorance, g.D., Fornasier, F., gondini, C.,
atientswithAISofditerentseverities were compared , andthecorrelationbetween serum OccludinlevelsandNIHSSscoresin p atientswith AIS was
as a p redictorofAISoccurrence , hemorrha g ictransformation and p oor p ro g nosis .
Keywords:Acuteischemicstroke ; Occludin; Bloodbrainbarrier
Both resistance training (RT) and long-duration, high-intensity stretching induce muscular adaptations; however, it is unknown whether the modalities are complementary or redundant, particularly in well-trained individuals. A case-study was conducted on a competitive bodybuilder implementing long-duration, high-intensity stretching of the plantar flexors (60 min 6x/week for 12 weeks) in conjunction with their habitual RT. Ultrasound muscle architecture (muscle thickness [MT], fascicle length [FL], and pennation angle [PA]) measurements were collected at multiple sites at four weekly baseline sessions, six (mid) and 12 (post1) weeks following the commencement of the intervention, and a week after the intervention (post2) while isometric strength and range of motion (RoM) were obtained once at baseline, mid, post1, and post2. 2SD band plots were constructed to determine meaningful changes in MT, FL, and PA from the four baseline measures while percentage and absolute change across each timepoint were calculated for all variables. From baseline to post 1, RoM, strength, and MT increased 25.9%, 11.4%, and 7.4%-23.4%, respectively, while four MT and two PA sites exceeded the threshold for meaningful change. The combined stretching and RT protocols resulted in flexibility, strength, and MT adaptations; however, findings should be generalized with caution given the case-study nature of our investigation.
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